拟南芥免疫受体 FLS2 的直接转录调控由乙烯依赖型转录因子 EIN3 和 EIL1 介导。
Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1.
机构信息
The Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom.
出版信息
Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14502-7. doi: 10.1073/pnas.1003347107. Epub 2010 Jul 27.
Abstract
In plant innate immunity, the leucine-rich repeat receptor kinase FLS2 recognizes the bacterial pathogen-associated molecular pattern (PAMP) flagellin. The molecular mechanisms underlying PAMP perception are not fully understood. Here, we reveal that the gaseous phytohormone ethylene is an integral part of PAMP-triggered immunity. Plants mutated in the key ethylene-signaling protein EIN2 are impaired in all FLS2-mediated responses, correlating with reduced FLS2 transcription and protein accumulation. The EIN3 and EIN3-like transcription factors, which depend on EIN2 activity for their accumulation, directly control FLS2 expression. Our results reveal a direct role for ethylene in regulation of an innate immune receptor.
摘要
在植物先天免疫中，富含亮氨酸重复受体激酶 FLS2 识别细菌病原体相关分子模式（PAMP）鞭毛蛋白。PAMP 感知的分子机制尚未完全了解。在这里，我们揭示了气态植物激素乙烯是 PAMP 触发免疫的一个组成部分。在关键的乙烯信号蛋白 EIN2 发生突变的植物中，所有 FLS2 介导的反应都受到损害，这与 FLS2 转录和蛋白积累减少有关。EIN3 和 EIN3 样转录因子依赖于 EIN2 的活性积累，它们直接控制 FLS2 的表达。我们的研究结果揭示了乙烯在调节先天免疫受体中的直接作用。
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2
Differential innate immune signalling via Ca(2+) sensor protein kinases.
Nature. 2010 Mar 18;464(7287):418-22. doi: 10.1038/nature08794. Epub 2010 Feb 17.
3
Rapid heteromerization and phosphorylation of ligand-activated plant transmembrane receptors and their associated kinase BAK1.
J Biol Chem. 2010 Mar 26;285(13):9444-9451. doi: 10.1074/jbc.M109.096842. Epub 2010 Jan 26.
4
A receptor-like cytoplasmic kinase, BIK1, associates with a flagellin receptor complex to initiate plant innate immunity.
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5
Network properties of robust immunity in plants.
PLoS Genet. 2009 Dec;5(12):e1000772. doi: 10.1371/journal.pgen.1000772. Epub 2009 Dec 11.
6
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Plant Signal Behav. 2009 Jul;4(7):672-4. doi: 10.1073/pnas.0810206106. Epub 2009 Jul 19.
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