National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Trends Cell Biol. 2010 Sep;20(9):503-6. doi: 10.1016/j.tcb.2010.06.008. Epub 2010 Jul 29.
The next revolution in microscopy is upon us: it is High-Throughput Imaging (HTI). In HTI large numbers of images from many samples are acquired and analyzed. This has become possible due to a confluence of dramatic progress in microscope engineering, enabling efficient image collection, and the availability of high computing power for data analysis. Combining HTI with genome-wide RNA interference (RNAi)-based gene knockdown technology offers a powerful approach for unbiased discovery of cellular mechanisms.
这就是高通量成像(HTI)。在 HTI 中,从许多样本中获取并分析大量图像。这之所以成为可能,是因为显微镜工程的巨大进步、高效图像采集能力以及用于数据分析的高计算能力的融合。将 HTI 与基于全基因组 RNA 干扰(RNAi)的基因敲低技术相结合,为无偏发现细胞机制提供了一种强大的方法。