通过延时显微镜对人类基因组进行表型分析揭示了细胞分裂基因。
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.
机构信息
MitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
出版信息
Nature. 2010 Apr 1;464(7289):721-7. doi: 10.1038/nature08869.
Abstract
Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the approximately 21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes. Phenotypes were scored quantitatively by computational image processing, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival. As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community.
摘要
尽管我们对人类基因组的了解迅速增长，但我们仍不知道一些最基本生命功能所需的所有基因。为了开始填补这一空白，我们开发了一种高通量表型筛选平台，该平台结合了 RNA 干扰的强大基因沉默、延时显微镜和计算图像处理。我们通过对荧光标记染色体进行为期两天的实时成像，对大约 21000 个人类蛋白编码基因进行了全基因组表型分析。通过计算图像处理对表型进行定量评分，使我们能够鉴定出数百个人类基因，这些基因涉及多种生物学功能，包括细胞分裂、迁移和存活。作为 Mitocheck 联盟的一部分，这项研究对细胞分裂表型进行了深入分析，并将整个高内涵数据集作为资源提供给社区。
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