Department of Biomolecular Functional Engineering, Faculty of Engineering, Ibaraki University, 4-12-1 Nakanarusawa, Hitachi, Ibaraki 316-8511, Japan.
J Anal Methods Chem. 2012;2012:520248. doi: 10.1155/2012/520248. Epub 2012 Mar 27.
The chemiluminescence (CL) signal immediately appeared when a hydrogen peroxide solution was injected into an iron-phthalocyanine tetrasulfonic acid (Fe-PTS) aqueous solution. Moreover, the CL intensity of Fe-PTS decreased by adding l-tyrosine. Based on these results, the determination of trace amounts of l-tyrosine was developed using the quenching-chemiluminescence. The calibration curve of l-tyrosine was obtained in the concentration range of 2.0 × 10(-7) M to 2.0 × 10(-5) M. Moreover, the relative standard deviation (RSD) was 1.63 % (n = 5) for 2.0 × 10(-6) M l-tyrosine, and its detection limits (3σ) were 1.81 × 10(-7) M. The spike and recovery experiments for l-tyrosine were performed using a soft drink. Furthermore, the determination of l-tyrosine was applied to supplements containing various kinds of amino acids. Each satisfactory relative recovery was obtained at 98 to 102%.
当过氧化氢溶液被注入铁酞菁四磺酸（Fe-PTS）水溶液中时，立即出现化学发光（CL）信号。此外，添加 l-酪氨酸会降低 Fe-PTS 的 CL 强度。基于这些结果，使用猝灭-化学发光法开发了痕量 l-酪氨酸的测定方法。在 2.0×10（-7）M 至 2.0×10（-5）M 的浓度范围内获得了 l-酪氨酸的校准曲线。此外，对于 2.0×10（-6）M l-酪氨酸，相对标准偏差（RSD）为 1.63%（n=5），检测限（3σ）为 1.81×10（-7）M。使用软饮料进行了 l-酪氨酸的加标和回收实验。此外，将 l-酪氨酸的测定应用于含有各种氨基酸的补充剂。在 98 到 102%的范围内获得了每个令人满意的相对回收率。
