Division of Health Research & Planning, Gyeonggi-do Research Institute of Health & Environment, Pajang-dong 324-1, Suwon, Gyeonggi-do, Republic of Korea.
J Microbiol Biotechnol. 2010 Jul;20(7):1107-13. doi: 10.4014/jmb.0912.12016.
Because conventional methods for detecting emetic-toxin-producing B. cereus are laborious and costly, various PCR assays, which are easy and cheap, have recently been reported. Therefore, this study estimated and compared the ability of various PCR assays to detect emetic-toxin-producing B. cereus strains isolated in Korea. The PCR assays were performed on 160 B. cereus strains, including 40 emetic-toxin-producing strains. Although the species-specific PCR assays were all shown to be highly specific, the sensitivities varied greatly. The accuracies of the primers were 97.5% (CER), 95.6% (EM1), 96.3% (RE234), 89.4% (CES), and 83.1% (Ces3R/CESR2). Moreover, the CER primer had a higher sensitivity (100%) than all the other primers tested, and a specificity of 96.7%. Thus, the CER primer was shown to be the most effective for screening the emetic-toxin-producing B. cereus strains tested in this study. However, the ability of these PCR assays to identify emetic-toxin-producing B. cereus should also be confirmed using other methods.
由于传统的呕吐毒素产生型 B. cereus 检测方法繁琐且昂贵，因此最近报道了各种简单且廉价的 PCR 检测方法。因此，本研究评估并比较了各种 PCR 检测方法对韩国分离的呕吐毒素产生型 B. cereus 菌株的检测能力。对包括 40 株产呕吐毒素菌株在内的 160 株 B. cereus 菌株进行了 PCR 检测。虽然各物种特异性 PCR 检测方法均显示出高度特异性，但灵敏度差异很大。引物的准确性分别为 97.5%（CER）、95.6%（EM1）、96.3%（RE234）、89.4%（CES）和 83.1%（Ces3R/CESR2）。此外，CER 引物的灵敏度（100%）高于所有其他测试引物，特异性为 96.7%。因此，CER 引物被证明是本研究中测试的呕吐毒素产生型 B. cereus 菌株筛选的最有效方法。然而，还应使用其他方法来确认这些 PCR 检测方法识别呕吐毒素产生型 B. cereus 的能力。
