Glasset Benjamin, Herbin Sabine, Guillier Laurent, Cadel-Six Sabrina, Vignaud Marie-Léone, Grout Joel, Pairaud Sylvie, Michel Valérie, Hennekinne Jacques-Antoine, Ramarao Nalini, Brisabois Anne
Université Paris-Est, ANSES, Laboratory for Food Safety, Maisons-Alfort Cedex, France.
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
Euro Surveill. 2016 Dec 1;21(48). doi: 10.2807/1560-7917.ES.2016.21.48.30413.
The aim of this study was to identify and characterise Bacillus cereus from a unique national collection of 564 strains associated with 140 strong-evidence food-borne outbreaks (FBOs) occurring in France during 2007 to 2014. Starchy food and vegetables were the most frequent food vehicles identified; 747 of 911 human cases occurred in institutional catering contexts. Incubation period was significantly shorter for emetic strains compared with diarrhoeal strains A sub-panel of 149 strains strictly associated to 74 FBOs and selected on Coliphage M13-PCR pattern, was studied for detection of the genes encoding cereulide, diarrhoeic toxins (Nhe, Hbl, CytK1 and CytK2) and haemolysin (HlyII), as well as panC phylogenetic classification. This clustered the strains into 12 genetic signatures (GSs) highlighting the virulence potential of each strain. GS1 (nhe genes only) and GS2 (nhe, hbl and cytK2), were the most prevalent GS and may have a large impact on human health as they were present in 28% and 31% of FBOs, respectively. Our study provides a convenient molecular scheme for characterisation of B. cereus strains responsible for FBOs in order to improve the monitoring and investigation of B. cereus-induced FBOs, assess emerging clusters and diversity of strains.
本研究的目的是从一个独特的国家菌株库中鉴定并表征蜡样芽孢杆菌，该菌株库包含564株与2007年至2014年期间在法国发生的140起有充分证据的食源性疾病暴发（FBOs）相关的菌株。淀粉类食物和蔬菜是最常见的食源载体；911例人类病例中有747例发生在集体餐饮环境中。与腹泻型菌株相比，呕吐型菌株的潜伏期明显更短。对149株与74起FBOs严格相关且根据噬菌体M13-PCR模式选择的菌株亚组进行了研究，以检测编码cereulide、腹泻毒素（Nhe、Hbl、CytK1和CytK2）和溶血素（HlyII）的基因，以及panC系统发育分类。这将菌株聚类为12个基因特征（GSs），突出了每个菌株的毒力潜力。GS1（仅含nhe基因）和GS2（含nhe、hbl和cytK2基因）是最普遍的GSs，分别存在于28%和31%的FBOs中，可能对人类健康有很大影响。我们的研究提供了一种便捷的分子方案，用于表征导致FBOs的蜡样芽孢杆菌菌株，以改进对蜡样芽孢杆菌引起的FBOs的监测和调查，评估新出现的菌株簇和菌株多样性。
