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Lett Appl Microbiol. 2017 Jul;65(1):57-65. doi: 10.1111/lam.12744. Epub 2017 May 30.
2
Evaluation of various PCR assays for the detection of emetic toxin producing Bacillus cereus.评价各种用于检测产吐毒素的蜡样芽胞杆菌的 PCR 检测方法。
J Microbiol Biotechnol. 2010 Jul;20(7):1107-13. doi: 10.4014/jmb.0912.12016.
3
Evaluation of Sanita-kun E. coli & coliform sheet medium for the enumeration of total coliforms and E. coli.用于总大肠菌群和大肠杆菌计数的Sanita-kun大肠杆菌和大肠菌群平板培养基的评估。
J AOAC Int. 2010 Jan-Feb;93(1):163-83.
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Cellular defenses against superoxide and hydrogen peroxide.细胞对超氧化物和过氧化氢的防御机制。
Annu Rev Biochem. 2008;77:755-76. doi: 10.1146/annurev.biochem.77.061606.161055.
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Structure and mechanism of the lactose permease.乳糖通透酶的结构与机制。
C R Biol. 2005 Jun;328(6):557-67. doi: 10.1016/j.crvi.2005.03.008.
6
Enumeration of heterotrophs, fecal coliforms and Escherichia coli in water: comparison of 3M Petrifilm plates with standard plating procedures.水中异养菌、粪大肠菌群和大肠杆菌的计数：3M Petrifilm平板与标准平板计数法的比较
J Microbiol Methods. 2005 Mar;60(3):335-42. doi: 10.1016/j.mimet.2004.10.008.
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Aeromonas isolates from human diarrheic stool and groundwater compared by pulsed-field gel electrophoresis.通过脉冲场凝胶电泳对从人类腹泻粪便和地下水中分离出的气单胞菌进行比较。
Emerg Infect Dis. 2003 Feb;9(2):224-8. doi: 10.3201/eid0902.020031.
8
Enumeration of coliforms and Escherichia coli in frozen black tiger shrimp Penaeus monodon by conventional and rapid methods.采用传统方法和快速方法对冷冻斑节对虾中的大肠菌群和大肠杆菌进行计数。
Int J Food Microbiol. 2003 Mar 15;81(2):113-21. doi: 10.1016/s0168-1605(02)00190-3.
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Diversity of properties among catalases.过氧化氢酶之间性质的多样性。
Arch Biochem Biophys. 2002 May 15;401(2):145-54. doi: 10.1016/S0003-9861(02)00049-8.
10
Detection and enumeration of coliforms in drinking water: current methods and emerging approaches.饮用水中大肠菌群的检测与计数：当前方法与新兴技术
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使用改良的干可复水膜法提高肉制品中大肠菌群检测的准确性。

Improving the accuracy of coliform detection in meat products using modified dry rehydratable film method.

作者信息

Kang Ju-Young, Lee Seo-Hyun, Jo Ah-Hyeon, Park Eun-Ji, Bak Young-Seok, Kim Jung-Beom

机构信息

Department of Food Science and Technology, Suncheon National University, Suncheon, 57922 Republic of Korea.

Department of Emergency Medical Services, Sun Moon University, Asan-si, Chungcheongnam-do, 31460 Republic of Korea.

出版信息

Food Sci Biotechnol. 2020 May 30;29(9):1289-1294. doi: 10.1007/s10068-020-00778-8. eCollection 2020 Sep.

DOI:10.1007/s10068-020-00778-8
PMID:32802568
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7406608/
Abstract

This study was undertaken to improve the detection accuracy for coliform bacteria, by analyzing biochemical properties of false positive and false negative colonies isolated from two dry rehydratable film methods, 3 M™ Petrifilm™ /Coliform count (PCC) and MC-Media Pad coliform count (MCC). The detection accuracy of PCC and MCC was determined to be 99.4% and 97.9%, respectively, with the detection error being 0.6% and 2.1%, respectively. False positive colonies (red colony without gas) on PCC were identified as and . All false positive colonies on MCC were identified as ; this organism gives a positive oxidase test, whereas coliform bacteria are oxidase negative. In conclusion, we propose that for improving detection accuracy of coliform bacteria, the incubation time of PCC should be modified and increased from 24 h to 48 h, and the oxidase test of MCC isolates should be included in the Korea Food Code.

摘要

本研究旨在通过分析从两种干式复水膜方法（3M™ Petrifilm™/大肠菌群计数（PCC）和MC-培养基平板大肠菌群计数（MCC））中分离出的假阳性和假阴性菌落的生化特性，提高大肠菌群的检测准确性。PCC和MCC的检测准确率分别为99.4%和97.9%，检测误差分别为0.6%和2.1%。PCC上的假阳性菌落（无气体的红色菌落）被鉴定为 和 。MCC上的所有假阳性菌落均被鉴定为 ；这种微生物氧化酶试验呈阳性，而大肠菌群氧化酶试验呈阴性。总之，我们建议，为提高大肠菌群的检测准确性，应将PCC的培养时间从24小时延长至48小时，并在《韩国食品法典》中纳入对MCC分离株的氧化酶试验。