Research Center for Applied Sciences, Academia Sinica 128, Section 2, Academia Road, Nangkang, Taipei 11529, Taiwan.
Small. 2010 Sep 6;6(17):1900-7. doi: 10.1002/smll.201000598.
A multispectral integration method to increase the detection limit of gold nanostructures is presented. This method considers all the resonances due to localized surface plasmons, Bloch wave surface plasmons, and Wood's anomalies. By integrating the wavelength shifts together with intensity changes over these resonances, the detection resolution is increased to about six times larger than that of commonly used wavelength or intensity methods. Further studies with different nanostructures show the detection sensitivity is increased with the decrease of aperture size. The detection limit for 40-nm nanoslits is improved by about seven times relative to that for 300-nm nanoslits. For sub-100-nm apertures, the detection resolution for nanoslits is better than that for nanoholes due to its non-cutoff transmission. The advantage of using the multispectral integration method in biosensing is verified by antigen-antibody interaction experiments.
提出了一种多光谱集成方法来提高金纳米结构的检测极限。该方法考虑了所有由于局域表面等离激元、布洛赫波表面等离激元和伍德反常引起的共振。通过将这些共振的波长移动与强度变化相结合，检测分辨率提高到比常用的波长或强度方法大约大六倍。对不同纳米结构的进一步研究表明，随着孔径尺寸的减小，检测灵敏度增加。与 300nm 纳米狭缝相比，40nm 纳米狭缝的检测极限提高了约 7 倍。对于小于 100nm 的孔径，由于其非截止传输，纳米狭缝的检测分辨率优于纳米孔。通过抗原-抗体相互作用实验验证了在生物传感中使用多光谱集成方法的优势。
