Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 10608, Taiwan.
Department of Vehicle Engineering, National Taipei University of Technology, Taipei 10608, Taiwan.
Sensors (Basel). 2023 May 31;23(11):5216. doi: 10.3390/s23115216.
Surface plasmon resonance (SPR) sensing is a real-time detection technique for measuring biomolecular interactions on gold surfaces. This study presents a novel approach using nano-diamonds (NDs) on a gold nano-slit array to obtain an extraordinary transmission (EOT) spectrum for SPR biosensing. We used anti-bovine serum albumin (anti-BSA) to bind NDs for chemical attachment to a gold nano-slit array. The covalently bound NDs shifted the EOT response depending on their concentration. The number of ND-labeled molecules attached to the gold nano-slit array was quantified from the change in the EOT spectrum. The concentration of anti-BSA in the 35 nm ND solution sample was much lower than that in the anti-BSA-only sample (approximately 1/100). With the help of 35 nm NDs, we were able to use a lower concentration of analyte in this system and obtained better signal responses. The responses of anti-BSA-linked NDs had approximately a 10-fold signal enhancement compared to anti-BSA alone. This approach has the advantage of a simple setup and microscale detection area, which makes it suitable for applications in biochip technology.
表面等离子体共振（SPR）感测是一种实时检测技术，用于测量金表面上的生物分子相互作用。本研究提出了一种新的方法，使用金纳米狭缝阵列上的纳米金刚石（NDs）获得 SPR 生物传感的非凡透射（EOT）光谱。我们使用抗牛血清白蛋白（anti-BSA）将 NDs 结合以进行化学附着到金纳米狭缝阵列上。根据其浓度，共价结合的 NDs 改变了 EOT 响应。从 EOT 光谱的变化可以定量出附着在金纳米狭缝阵列上的 ND 标记分子的数量。在 35nm ND 溶液样品中，anti-BSA 的浓度比仅含有 anti-BSA 的样品低得多（约为 1/100）。借助 35nm NDs，我们能够在该系统中使用较低浓度的分析物，并获得更好的信号响应。与单独的 anti-BSA 相比，anti-BSA 连接的 NDs 的响应信号增强了约 10 倍。这种方法具有简单的设置和微尺度检测区域的优势，使其适用于生物芯片技术的应用。
