Department of Cardiology, Peking University First Hospital, Beijing, China.
J Zhejiang Univ Sci B. 2010 Aug;11(8):583-91. doi: 10.1631/jzus.B1001003.
To investigate the effect of enalapril on plasma homocysteine (Hcy) levels and the association of methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism with the changes of Hcy levels in response to enalapril among patients with essential hypertension.
A total of 130 patients with mild-to-moderate essential hypertension were enrolled and enalapril was orally administered at a dose of 10 mg/d for eight weeks. Plasma Hcy levels were measured by denaturing high-performance liquid chromatography (DHPLC) at baseline and after eight weeks of treatment. Genotyping of MTHFR C677T polymorphism was performed by TaqMan probe technique.
Compared with baseline, plasma Hcy levels did not change significantly after eight weeks (P=0.81). Stratified by baseline Hcy levels, a significant increase in plasma Hcy levels (P=0.02) among those with Hcy <10 micromol/L was observed, in contrast to no significant changes in plasma Hcy levels (P=0.54) among those with Hcy > or =10 micromol/L. No significant association was observed between MTHFR C677T polymorphism and changes in Hcy levels in response to enalapril.
Enalapril may cause an increase in plasma Hcy levels among the hypertensives with low baseline Hcy levels. There was no significant association between MTHFR C677T genotypes and changes in Hcy levels in response to enalapril among subjects with essential hypertension.
研究依那普利对血浆同型半胱氨酸（Hcy）水平的影响，以及亚甲基四氢叶酸还原酶（MTHFR）C677T 多态性与依那普利治疗后 Hcy 水平变化的相关性在原发性高血压患者中的作用。
共纳入 130 例轻中度原发性高血压患者，给予依那普利 10mg/d 口服治疗 8 周。采用变性高效液相色谱法（DHPLC）在基线和治疗 8 周时测定血浆 Hcy 水平。采用 TaqMan 探针技术检测 MTHFR C677T 多态性。
与基线相比，8 周后血浆 Hcy 水平无明显变化（P=0.81）。按基线 Hcy 水平分层，Hcy<10μmol/L 者血浆 Hcy 水平显著升高（P=0.02），而 Hcy≥10μmol/L 者血浆 Hcy 水平无明显变化（P=0.54）。MTHFR C677T 多态性与依那普利治疗后 Hcy 水平变化之间无显著相关性。
依那普利可能导致基线 Hcy 水平较低的高血压患者血浆 Hcy 水平升高。原发性高血压患者中，MTHFR C677T 基因型与依那普利治疗后 Hcy 水平变化之间无显著相关性。
