Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
Inflamm Res. 2011 Dec;60(12):1131-8. doi: 10.1007/s00011-011-0377-7. Epub 2011 Aug 30.
The aim of this study was to evaluate the effect of sulfated glucosamine (SGlc) on the regulation of inflammatory cytokines and profiles involved in immunological activities. Changes in the inflammatory profiles of lipopolysaccharide (LPS)-activated phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 macrophage models were investigated following SGlc treatment.
Human THP-1 macrophages were used to evaluate anti-inflammatory profiles. The cytokine secretion levels were measured by enzyme-linked immunosorbent assay (ELISA). Effects of SGlc on the regulation of mRNA and protein levels were determined using RT-PCR and Western blot analysis. The effect of SGlc on the activation of mitogen-activated protein kinases (MAPKs) and NF-κB protein was also determined by Western blot analysis.
Treatment of THP-1 cells with SGlc inhibited the production of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6. In addition, SGlc suppressed the mRNA and protein expression levels of inflammatory mediators such as inducible nitric oxide synthase, cyclooxygenase-2, TNF-α, IL-1β, IL-6, 5-lipoxygenase and cytoplasmic phospholipase A(2) in LPS-activated THP-1 macrophages. Furthermore, we confirmed that the LPS-activated transcriptions of MAPKs and NF-κB were inhibited by SGlc treatment in PMA-differentiated THP-1 macrophages.
These results suggest that SGlc can be considered as a potential anti-inflammatory supplement.
本研究旨在评估硫酸氨基葡萄糖（SGlc）对调节参与免疫活性的炎症细胞因子和谱的影响。在 SGlc 处理后，研究了 LPS 激活的佛波醇 12-肉豆蔻酸 13-乙酸酯（PMA）分化的 THP-1 巨噬细胞模型中炎症谱的变化。
使用人 THP-1 巨噬细胞评估抗炎谱。通过酶联免疫吸附测定（ELISA）测量细胞因子分泌水平。使用 RT-PCR 和 Western blot 分析确定 SGlc 对 mRNA 和蛋白水平调节的影响。还通过 Western blot 分析确定 SGlc 对丝裂原活化蛋白激酶（MAPKs）和 NF-κB 蛋白激活的影响。
SGlc 处理 THP-1 细胞可抑制促炎细胞因子如肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β和 IL-6 的产生。此外，SGlc 抑制了 LPS 激活的 THP-1 巨噬细胞中炎症介质如诱导型一氧化氮合酶、环氧化酶-2、TNF-α、IL-1β、IL-6、5-脂氧合酶和细胞质磷脂酶 A（2）的 mRNA 和蛋白表达水平。此外，我们证实 SGlc 处理抑制了 LPS 激活的 PMA 分化的 THP-1 巨噬细胞中 MAPKs 和 NF-κB 的转录。
这些结果表明 SGlc 可被视为一种潜在的抗炎补充剂。
