Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, Rockville, Maryland 20852, USA.
J Bacteriol. 2010 Oct;192(19):5245-8. doi: 10.1128/JB.00633-10. Epub 2010 Jul 30.
Group IIC introns insert next to the stem-loop structure of rho-independent transcription terminators, thus avoiding intact genes. The insertion sites of 17 copies of the G.st.I1 intron from Geobacillus stearothermophilus were compared. One copy of the intron was found to interrupt an open reading frame (ORF) encoding an rRNA methylase.
IIC 组内含子插入到 rho 独立转录终止子的茎环结构旁边,从而避开完整的基因。比较了来自嗜热脂肪地芽孢杆菌的 17 个 G.st.I1 内含子的插入位点。一个内含子被发现中断了一个编码 rRNA 甲基酶的开放阅读框(ORF)。