Department of Gastroenterology, 309 Hospital of PLA, Beijing, China.
World J Gastroenterol. 2010 Aug 7;16(29):3664-73. doi: 10.3748/wjg.v16.i29.3664.
To investigate and elucidate the molecular mechanism underlying varioliform gastritis for early detection, prevention and intervention of gastric cancer.
A combination of two-dimensional gel electrophoresis and mass spectrometry was used to detect the differentially expressed proteins between varioliform gastritis and matched normal mucosa. The selected proteins were confirmed by Western blotting and reverse transcription polymerase chain reaction (RT-PCR) in additional samples and the function of some proteins in varioliform gastritis was analyzed by bio-method preliminarily.
We identified 21 differentially expressed proteins in varioliform gastritis, and compared them with matched normal mucosa. Eleven proteins were upregulated and ten downregulated in varioliform gastritis when compared with the same proteins in individual-matched normal gastric mucosa. These proteins are related to metabolism, oxidation, cytoskeleton, apoptosis, signal transduction and other aspects of cells. Two novel proteins, thioredoxin domain-containing protein 5 (TXNDC5) upregulated in varioliform gastritis, and neuropolypeptide h3 [phosphatidylethanolamine-binding protein 1 (PEBP1)] downregulated in varioliform gastritis, were further investigated. Their expressions were validated by Western blotting and RT-PCR in 12 cases of varioliform gastritis which was matched with normal mucosa. The expression level of PEBP1 in varioliform gastritis was significantly lower (P < 0.05) while that of TXNDC5 was significantly higher than that in matched normal gastric mucosa (P < 0.05).
There are some changes of protein expression in varioliform gastritis. Downregulation of PEBP1 and upregulation of TXNDC5 are involved in the development of varioliform gastritis.
研究阐明天花样胃炎的分子机制，以期实现胃癌的早期检测、预防和干预。
采用二维凝胶电泳和质谱联用技术检测天花样胃炎与配对正常黏膜之间差异表达的蛋白质，选择部分差异表达蛋白，采用 Western blot 和逆转录聚合酶链反应（RT-PCR）方法在更多样本中验证，并初步采用生物方法分析部分蛋白在天花样胃炎中的作用。
我们在天花样胃炎中鉴定出 21 种差异表达蛋白，并与配对的正常黏膜进行比较。与个体配对的正常胃黏膜中的相同蛋白相比，天花样胃炎中有 11 种蛋白上调，10 种蛋白下调。这些蛋白与细胞的代谢、氧化、细胞骨架、凋亡、信号转导等方面有关。进一步研究了 2 种在天花样胃炎中上调的新型蛋白，即硫氧还蛋白结构域蛋白 5（TXNDC5）和下调的神经多肽 h3[磷酸乙醇胺结合蛋白 1（PEBP1）]。在 12 例天花样胃炎及其配对的正常黏膜中，采用 Western blot 和 RT-PCR 验证了它们的表达。天花样胃炎中 PEBP1 的表达水平显著降低（P < 0.05），而 TXNDC5 的表达水平显著升高（P < 0.05）。
天花样胃炎存在某些蛋白质表达的变化，PEBP1 下调和 TXNDC5 上调参与了天花样胃炎的发生发展。
