Rastovac M, Janković Z
Institute of Nuclear Medicine, Military Medical Academy, Belgrade, Yugoslavia.
Nucl Med Commun. 1991 May;12(5):461-4. doi: 10.1097/00006231-199105000-00011.
A modified method of in vitro labelling of red blood cells with 99Tcm using whole blood is presented. Blood samples from patients with normal blood counts were used. First, the blood sample was exposed to a low concentration of stannous ions (0.02-0.4 micrograms/2 ml blood), citrate phosphate glucose solution was then added followed by 99Tcm pertechnetate. A high labelling yield of greater than 90% was achieved in a maximum of 30 min.
介绍了一种使用全血对红细胞进行99锝体外标记的改良方法。使用了血细胞计数正常患者的血样。首先,将血样暴露于低浓度的亚锡离子(0.02 - 0.4微克/2毫升血液)中,然后加入枸橼酸盐 - 磷酸盐 - 葡萄糖溶液,接着加入高锝酸盐。在最多30分钟内实现了大于90%的高标记率。