Bernardo-Filho M, Gutfilen B, Maciel O de S
Universidade do Estado do Rio de Janeiro, Departamento de Biofísica e Biometria, Brasil.
Nucl Med Commun. 1994 Sep;15(9):730-4. doi: 10.1097/00006231-199409000-00011.
There are controversies about the effect of different anticoagulants on the labelling of blood elements with 99Tcm. Our results show that the type of anticoagulant employed to withdraw the whole blood can modify the 99Tcm labelling of red blood cells (RBC) and plasma proteins (PP). The anticoagulants ACD (citric acid, sodium citrate and dextrose solution), heparin and sodium oxalate present similar results for the 99Tcm labelling of RBC with the exception of 0.13 microM stannous chloride. In this assay oxalate provides the best RBC labelling. In addition, with ethylenediaminetetraacetic acid (EDTA) the labelling of RBC is almost always lower than with the other anticoagulants, probably due to its high chelating capacity. The anticoagulants ACD, oxalate and heparin show the same results as expected with 99Tcm labelling of PP. The lowest labelling at 13.00 microM stannous chloride in the presence of oxalate is probably due to its low chelating capacity. The results also reinforce the idea that the erythrocyte membrane exerts an important role in the regulation of stannous ion transport into RBCs.
关于不同抗凝剂对用99Tcm标记血液成分的影响存在争议。我们的结果表明，用于采集全血的抗凝剂类型可改变红细胞（RBC）和血浆蛋白（PP）的99Tcm标记。除了0.13微摩尔氯化亚锡外，抗凝剂ACD（柠檬酸、柠檬酸钠和葡萄糖溶液）、肝素和草酸钠在RBC的99Tcm标记方面呈现相似的结果。在该实验中，草酸盐能提供最佳的RBC标记。此外，使用乙二胺四乙酸（EDTA）时，RBC的标记几乎总是低于其他抗凝剂，这可能是由于其高螯合能力所致。抗凝剂ACD、草酸盐和肝素在PP的99Tcm标记方面显示出预期的相同结果。在草酸盐存在的情况下，13.00微摩尔氯化亚锡时标记最低，这可能是由于其低螯合能力。结果还强化了红细胞膜在调节亚锡离子转运到RBC中起重要作用这一观点。
