[宣威女性肺癌患者肺组织中PAH-DNA加合物的表达]

[Expression of PAH-DNA adducts in lung tissues of Xuanwei female lung cancer patients].

作者信息

Yang Kaiyun, Huang Yunchao, Zhao Guangqiang, Lei Yujie, Wang Kun

机构信息

Department of Thoracic Cardiovascular Surgery, Yunnan Tumor Hospital (the Third Affiliated Hospital of Kunming Medical College), Kunming 650118, China.

出版信息

Zhongguo Fei Ai Za Zhi. 2010 May;13(5):517-21. doi: 10.3779/j.issn.1009-3419.2010.05.25.

DOI:10.3779/j.issn.1009-3419.2010.05.25

PMID:20677652

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6000705/

Abstract

BACKGROUND AND OBJECTIVE

The coal-fired pollution in Xuanwei area has been considered to be local main reason for high incidence of female lung cancer. The aim of this study is to explore the expression of PAH-DNA adducts in lung tissues of Xuanwei female lung cancer patients and to explore the relationship between the large number of coal-fired pollution PAHs materials and the high incidence of Xuanwei female lung cancer.

METHODS

We totally collected each 20 cases of Xuanwei female lung cancer patients, Xuanwei male lung cancer patients, Non-Xuanwei female lung cancer patients and collect each 10 cases of Xuanwei, Non-Xuanwei female patients with benign lung lesions. The cancer tissues, adjacent cancer tissues and normal lung tissues were collected in lung cancer patients and only the normal tissues were collected in benign lung lesion patients. There were total 80 cases and 200 tissues. Immunofluorescence was used to detect the expression of PAH-DNA adducts in each group. Image pro-plus 6.0 software was used to analyze the images and part quantified analysis. SPSS 13.0 statistical software was used to analyze the data.

RESULTS

The positive expression of PAH-DNA adducts in lung cancer tissues, adjacent cancer tissues and normal lung tissues of Xuanwei female lung cancer patients were 90%, 80% and 65%. They were higher than the positive expression of PAH-DNA adducts in Xuanwei male lung cancer patients (35%, 30%, 30%) and Non-Xuanwei female lung cancer patients (20%, 15%, 10%) (P < 0.01). The expressions in lung tissues ofXuanwei female benign lung lesion patients (positive expression is 70%) were higher than it in Non-Xuanwei female benign lung lesion patients (positive expression is 10%). With the direction changing from cancer tissues, adjacent cancer tissues to normal lung tissues, the expressions of PAH-DNA adducts were decreased but had no statistical difference (P > 0.05S).

CONCLUSION

The expressions of PAH-DNA adducts in lung tissues of Xuanwei female were higher than which in Xuanwei male and Non-Xuanwei female.

摘要

背景与目的

宣威地区燃煤污染被认为是当地女性肺癌高发的主要原因。本研究旨在探讨宣威女性肺癌患者肺组织中多环芳烃 - DNA加合物的表达情况，并探究大量燃煤污染的多环芳烃物质与宣威女性肺癌高发之间的关系。

方法

我们共收集了宣威女性肺癌患者、宣威男性肺癌患者、非宣威女性肺癌患者各20例，以及宣威、非宣威女性良性肺病变患者各10例。肺癌患者收集癌组织、癌旁组织和正常肺组织，良性肺病变患者仅收集正常组织。共80例，200个组织。采用免疫荧光法检测各组中多环芳烃 - DNA加合物的表达。使用Image pro-plus 6.0软件分析图像并进行部分定量分析。采用SPSS 13.0统计软件分析数据。

结果

宣威女性肺癌患者的癌组织、癌旁组织和正常肺组织中多环芳烃 - DNA加合物的阳性表达率分别为90%、80%和65%。高于宣威男性肺癌患者（35%、30%、30%）和非宣威女性肺癌患者（20%、15%、10%）（P < 0.01）。宣威女性良性肺病变患者肺组织中的表达（阳性表达率为70%）高于非宣威女性良性肺病变患者（阳性表达率为10%）。随着从癌组织、癌旁组织到正常肺组织方向变化，多环芳烃 - DNA加合物的表达降低，但无统计学差异（P > 0.05）。