School of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju-si 690-756, South Korea.
Cell Biol Toxicol. 2011 Apr;27(2):83-94. doi: 10.1007/s10565-010-9172-4. Epub 2010 Aug 2.
The radioprotective effect of geraniin, a tannin compound isolated from Nymphaea tetragona Georgi var. (Nymphaeaceae), against γ-radiation-induced damage was investigated in Chinese hamster lung fibroblast (V79-4) cells. Geraniin recovered cell viability detected by MTT test and colony formation assay, which was compromised by γ-radiation, and reduced the γ-radiation-induced apoptosis by the inhibition of loss of the mitochondrial membrane potential. Geraniin protected cellular components (lipid membrane, cellular protein, and DNA) damaged by γ-radiation, which was detected by lipid peroxidation, protein carbonyl formation, and comet assay. Geraniin significantly reduced the level of intracellular reactive oxygen species generated by γ-radiation, which was detected using spectrofluorometer, flow cytometer, and confocal microscope after 2',7'-dichlorodihydrofluorescein diacetate staining. Geraniin normalized the superoxide dismutase and catalase activities, which were decreased by γ-radiation. These results suggest that geraniin protects cells against radiation-induced oxidative stress via enhancing of antioxidant enzyme activities and attenuating of cellular damage.
从睡莲科植物荷花的叶中分离得到的单宁化合物棓单宁对γ射线辐射损伤的放射保护作用。MTT 试验和集落形成试验检测到棓单宁恢复了受γ射线辐射损伤而降低的细胞活力，并通过抑制线粒体膜电位丧失来减少γ射线辐射诱导的细胞凋亡。棓单宁保护了脂质膜、细胞蛋白和 DNA 等细胞成分免受γ射线辐射损伤，这可以通过脂质过氧化、蛋白羰基形成和彗星试验检测到。棓单宁通过 2',7'-二氯二氢荧光素二乙酸酯染色后用分光荧光计、流式细胞仪和共聚焦显微镜检测到的，显著降低了γ射线辐射产生的细胞内活性氧物种的水平。棓单宁使超氧化物歧化酶和过氧化氢酶的活性正常化，这些酶的活性因γ射线辐射而降低。这些结果表明，棓单宁通过增强抗氧化酶的活性和减轻细胞损伤来保护细胞免受辐射诱导的氧化应激。
