Department of Biotechnology, Division of Applied Chemistry and Biotechnology, Hanbat National University, Daejeon 305-719, Korea.
Mol Cells. 2010 Aug;30(2):155-9. doi: 10.1007/s10059-010-0101-4. Epub 2010 Jul 23.
In the central nervous system (CNS), giving rise to the diversity and the complexity of neurons is the spatial and temporal differentiation of neural stem cells and/or neural precursors. Here, we investigated the role of Jagged-mediated Notch signaling in the maintenance and differentiation of progenitor cells during late neurogenesis by analyzing the expression patterns of zebrafish jagged homologues, and by injecting their morpholinos. Expression of both jagged2 and jagged1b mRNA in the CNS suggested that they might be involved in control of differentiating neural progenitors in which they are involved later in development. In Jagged2 and Jagged1b knock-down embryos, the overall rate of cell division dramatically decreased, and the ectopic VeMe neurons were generated. The results suggest that Jagged-Notch signaling plays a critical role in the maintenance of proliferating neural precursors, and that the generation of late-born neurons, especially VeMe neurons, is regulated by the interplay between Jagged2 and Jagged1b.
在中枢神经系统（CNS）中，神经干细胞和/或神经前体细胞的空间和时间分化导致神经元的多样性和复杂性。在这里，我们通过分析斑马鱼 jagged 同源物的表达模式，并通过注射它们的形态发生素来研究 Jagged 介导的 Notch 信号在晚期神经发生过程中祖细胞的维持和分化中的作用。CNS 中 jagged2 和 jagged1b mRNA 的表达表明它们可能参与控制分化中的神经祖细胞，而在发育后期它们参与其中。在 Jagged2 和 Jagged1b 敲低胚胎中，细胞分裂的整体速度显著降低，并且产生了异位的 VeMe 神经元。结果表明 Jagged-Notch 信号在维持增殖中的神经前体细胞中发挥关键作用，并且晚期出生的神经元的产生，特别是 VeMe 神经元，受 Jagged2 和 Jagged1b 之间的相互作用调节。
