U. S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.
Mycopathologia. 2011 Feb;171(2):133-8. doi: 10.1007/s11046-010-9352-z. Epub 2010 Aug 1.
The metabolic activity of the aflatoxigenic fungus, Aspergillus flavus co-cultured with the biocontrol yeast, Pichia anomala was examined using several viability stains. Both the FUN-1 stain and the combined use of DiBAC(4)(5) with CDFA-AM stains were applied in this study. The results suggest that the ATP-generating system in A. flavus was inactivated as the ratio of yeasts to fungi increased in the dual culture. A decrease in hyphal membrane potential and esterase activity was substantiated by the combined stains of DiBAC(4)(5) and CDFA-AM. Reduced metabolic function in conjunction with cell wall damage of A. flavus hindered the growth and biomass production of this fungus. Viability stains such as FUN-1 and DiBAC(4)(5) with CDFA-AM may assist in elucidating the biocontrol mechanism by allowing for the visualization of the antagonistic effect of yeast species on target fungi in situ, as well as for screening potent biocontrol yeast agents against fungal pathogens.
采用几种活细胞染色剂研究了与生防酵母酒香酵母共培养的产黄曲霉素真菌黄曲霉的代谢活性。本研究应用了 FUN-1 染色剂和 DiBAC（4）（5）与 CDFA-AM 联合染色剂。结果表明，随着双培养中酵母与真菌比例的增加，黄曲霉的 ATP 生成系统失活。DiBAC（4）（5）和 CDFA-AM 的联合染色证实了菌丝膜电位和酯酶活性的下降。黄曲霉代谢功能的降低以及细胞壁的损伤阻碍了该真菌的生长和生物量的产生。FUN-1 和 DiBAC（4）（5）与 CDFA-AM 等活细胞染色剂可用于阐明生防机制，因为它们可以直观地观察到酵母对目标真菌的拮抗作用，还可以筛选针对真菌病原体的有效生防酵母剂。
