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核基质蛋白F/G的分子克隆：一种含有假定锌指基序的核基质DNA结合蛋白。

Molecular cloning of matrin F/G: A DNA binding protein of the nuclear matrix that contains putative zinc finger motifs.

作者信息

Hakes D J, Berezney R

机构信息

Department of Biological Sciences, State University of New York, Buffalo 14260.

出版信息

Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6186-90. doi: 10.1073/pnas.88.14.6186.

DOI:10.1073/pnas.88.14.6186
PMID:2068100
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC52047/
Abstract

We have isolated a 2.7-kilobase rat liver cDNA clone that contains the entire 544-amino acid coding sequence for matrin F/G. This protein has previously been localized to the internal, fibrogranular areas of the nuclear matrix and shown to bind to DNA on nitrocellulose blots. The predicted amino acid sequence from the coding region of this cDNA shows that this protein contains approximately 50% hydrophobic amino acids with secondary structure predictions suggesting a large percentage of beta-sheet regions. No significant homologies were found with any other known proteins, including the nuclear lamins. The predicted amino acid sequence was also searched for DNA binding motifs. Two putative zinc finger motifs were found. In addition, a 7-mer palindromic sequence (Ser-Ser-Thr-Asn-Thr-Ser-Ser) was discovered within one of these zinc finger DNA binding regions. A possible regulatory role for this element is discussed.

摘要

我们分离出了一个2.7千碱基的大鼠肝脏cDNA克隆，它包含了基质蛋白F/G完整的544个氨基酸的编码序列。该蛋白先前已定位到核基质的内部纤维颗粒区域，并在硝酸纤维素印迹上显示出与DNA结合。从这个cDNA编码区预测的氨基酸序列表明，该蛋白含有大约50%的疏水氨基酸，二级结构预测显示有很大比例的β-折叠区域。未发现与任何其他已知蛋白（包括核纤层蛋白）有显著同源性。还在预测的氨基酸序列中搜索了DNA结合基序。发现了两个假定的锌指基序。此外，在其中一个锌指DNA结合区域内发现了一个7聚体回文序列（丝氨酸-丝氨酸-苏氨酸-天冬酰胺-苏氨酸-丝氨酸-丝氨酸）。讨论了该元件可能的调节作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d4a/52047/7dddeaa2c60b/pnas01064-0258-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d4a/52047/5ad228072309/pnas01064-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d4a/52047/ebf760370923/pnas01064-0258-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d4a/52047/7dddeaa2c60b/pnas01064-0258-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d4a/52047/5ad228072309/pnas01064-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d4a/52047/ebf760370923/pnas01064-0258-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d4a/52047/7dddeaa2c60b/pnas01064-0258-c.jpg

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