Tseng H, Green H
Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, MA 02115.
Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10311-5. doi: 10.1073/pnas.89.21.10311.
A cDNA clone has been prepared from mRNA of cultured human keratinocytes. The sequence of the cDNA reveals that in the C-terminal two-thirds of the corresponding protein (basonuclin), there are three separated pairs of adjacent zinc fingers. The amino acid sequence of each pair is homologous to that of the single pair of zinc fingers of the Drosophila transcription factor encoded by disco. Near the C-terminal end of basonuclin and on the surface of a putative alpha-helix, there is a stripe of serine residues similar to that of the transcription factor PRDII-BF1. Basonuclin possesses a sequence of six amino acids quite similar to one present in the myogenic family of proteins, including Myf5; this sequence is located in the omega loop of the myogenic proteins but within a zinc finger of the keratinocyte protein. As basonuclin is present mainly in the nuclei of the basal cell layer, its regulatory function is likely to be exerted prior to the process of terminal differentiation.
已从培养的人角质形成细胞的mRNA制备了一个cDNA克隆。该cDNA的序列显示，在相应蛋白质（锌指蛋白）的C端三分之二区域，有三对分开的相邻锌指。每对锌指的氨基酸序列与果蝇转录因子disco编码的单对锌指的氨基酸序列同源。在锌指蛋白的C端附近和假定的α螺旋表面，有一条丝氨酸残基带，类似于转录因子PRDII-BF1的丝氨酸残基带。锌指蛋白具有一段由六个氨基酸组成的序列，与包括Myf5在内的肌源性蛋白家族中的一段序列非常相似；该序列位于肌源性蛋白的ω环中，但在角质形成细胞蛋白的一个锌指内。由于锌指蛋白主要存在于基底细胞层的细胞核中，其调节功能可能在终末分化过程之前发挥作用。
