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聚合酶链反应(PCR)检测北非阿尔及利亚家犬巴尔通体感染的分子证据。

Molecular evidence of Bartonella infection in domestic dogs from Algeria, North Africa, by polymerase chain reaction (PCR).

机构信息

Département de zoologie, Institut National d'Agronomie, El Harrach, Algiers, Algeria.

出版信息

Am J Trop Med Hyg. 2010 Aug;83(2):298-300. doi: 10.4269/ajtmh.2010.09-0052.

DOI:10.4269/ajtmh.2010.09-0052
PMID:20682871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2911174/
Abstract

Bartonella species are being recognized as important bacterial human and canine pathogens, and are associated with multiple arthropod vectors. Bartonella DNA extracted from blood samples was obtained from domestic dogs in Algiers, Algeria. Polymerase chain reaction (PCR) and DNA sequence analyses of the ftsZ gene and the 16S-23S intergenic spacer region (ITS) were performed. Three Bartonella species: Bartonella vinsonii subsp. berkhoffii, Bartonella clarridgeiae, and Bartonells elizabethae were detected infecting Algerian dogs. To our knowledge, this study is the first report of detection by PCR amplification of Bartonella in dogs in North Africa.

摘要

贝氏巴尔通体种被认为是重要的人及犬类细菌病原体,与多种节肢动物媒介有关。从阿尔及利亚阿尔及尔的犬血样中提取贝氏巴尔通体 DNA。对 ftsZ 基因和 16S-23S 基因间隔区(ITS)进行聚合酶链反应(PCR)和 DNA 序列分析。检测到感染阿尔及利亚犬的三种贝氏巴尔通体:贝氏巴尔通体 berkhoffii 亚种、克拉氏巴尔通体和伊丽莎白巴尔通体。据我们所知,这项研究首次报告了在北非通过 PCR 扩增检测犬中的巴尔通体。

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