Department of Chemistry and Center for Bioactive Molecular Hybrids, Yonsei University, 134 Shinchon-dong, Seodaemoon-Gu, Seoul 120-749, Republic of Korea.
Biosens Bioelectron. 2010 Dec 15;26(4):1326-31. doi: 10.1016/j.bios.2010.07.038. Epub 2010 Jul 17.
A highly sensitive electrochemical lectin biosensor has been developed for the first time using carbohydrate-stabilized gold nanoparticles and silver-enhancement technique. A target lectin protein, Concanavalin A (Con A), was specifically bound to the self-assembled monolayer of thiolated mannose on a gold electrode. Mannose-stabilized gold nanoparticles were added to form a sandwich-type complex with the Con A and were followed by silver-enhancement process to coat the mannose-stabilized gold nanoparticles with silver metal. The coated metallic silver was dissolved in an acidic solution and the resulting silver ions were detected by anodic stripping voltammetry. The present lectin biosensor gave a linear response (R(2)=0.999) for Con A concentration from 0.084 μg/mL to 50.0 μg/mL with a remarkable detection limit (S/N=3) of 0.070 μg/mL, which is much lower compared to those obtained with the reported microgravimetric and colorimetric detection methods.
首次开发了一种基于糖基稳定金纳米粒子和银增强技术的高灵敏度电化学凝集素生物传感器。目标凝集素蛋白，刀豆球蛋白 A（Con A），特异性地结合到金电极上硫醇化甘露糖的自组装单层上。加入甘露糖稳定的金纳米粒子与 Con A 形成三明治型复合物，然后进行银增强处理，在甘露糖稳定的金纳米粒子上涂覆银金属。涂覆的金属银溶解在酸性溶液中，并通过阳极溶出伏安法检测产生的银离子。该凝集素生物传感器对 Con A 浓度在 0.084 μg/mL 至 50.0 μg/mL 范围内呈现线性响应（R(2)=0.999），具有显著的检测限（S/N=3）为 0.070 μg/mL，与报道的微量重量法和比色检测方法相比，检测限低得多。
