Jiangsu Provincial Key Lab of Organic Solid Waste Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Appl Microbiol Biotechnol. 2013 Nov;97(21):9479-89. doi: 10.1007/s00253-013-5157-6. Epub 2013 Sep 27.
Fusaricidins produced by Paenibacillus polymyxa are lipopeptide antibiotics with outstanding antifungal activity. In this study, the whole gene cluster responsible for fusaricidin biosynthesis (fusA) was isolated and identified from the cDNA library of one biocontrol agent P. polymyxa SQR-21 (SQR-21). MALDI-TOF MS analysis confirmed that SQR-21 could produce four kinds of fusaricidins: A, B, C, and D. A central promoter that drove the transcription of fusGFEDCBA was revealed by mapping of the fus promoter region by 5' deletions. The disruption of fusA in SQR-21 led to the abolishment of fusaricidin production and antifungal activity. The direct interaction between a potential regulator, AbrB, and the promoter region of fus gene cluster was confirmed by electrophoretic mobility shift assays. One abrB disruption mutant showed significantly higher antifungal activity compared with the wild type. These results revealed a pathway for the transcriptional regulation of the fus gene cluster in P. polymyxa.
多粘类芽孢杆菌产生的 Fusaricidins 是具有出色抗真菌活性的脂肽抗生素。在本研究中，从一种生防剂多粘类芽孢杆菌 SQR-21（SQR-21）的 cDNA 文库中分离并鉴定了负责 Fusaricidin 生物合成的完整基因簇（fusA）。 MALDI-TOF MS 分析证实 SQR-21 可以产生四种 Fusaricidins：A、B、C 和 D。通过 5' 缺失映射 Fus 启动子区域，揭示了驱动 fusGFEDCBA 转录的中央启动子。SQR-21 中 fusA 的缺失导致 Fusaricidin 产生和抗真菌活性的丧失。通过电泳迁移率变动分析证实了一个潜在调节剂 AbrB 与 fus 基因簇启动子区域之间的直接相互作用。一个 abrB 缺失突变体显示出比野生型更高的抗真菌活性。这些结果揭示了多粘类芽孢杆菌中 fus 基因簇转录调控的途径。
