Center for Magnetic Resonance Research and Department of Radiology, University of Minnesota, 2021 6th ST SE, Minneapolis, MN 55455, United States.
J Magn Reson. 2010 Oct;206(2):210-8. doi: 10.1016/j.jmr.2010.07.006. Epub 2010 Jul 16.
The low sensitivity of 13C spectroscopy can be enhanced using dynamic nuclear polarization. Detection of hyperpolarized [1-(13)C]pyruvate and its metabolic products has been reported in kidney, liver, and muscle. In this work, the feasibility of measuring 13C signals of hyperpolarized 13C metabolic products in the rat brain in vivo following the injection of hyperpolarized [1-(13)C]pyruvate and [2-(13)C]pyruvate is investigated. Injection of [2-(13)C]pyruvate led to the detection of [2-(13)C]lactate, but no other downstream metabolites such as TCA cycle intermediates were detected. Injection of [1-(13)C]pyruvate enabled the detection of both [1-(13)C]lactate and [13C]bicarbonate. A metabolic model was used to fit the hyperpolarized 13C time courses obtained during infusion of [1-(13)C]pyruvate and to determine the values of VPDH and VLDH.
13C 波谱的低灵敏度可以通过动态核极化来增强。已经有报道称，在肾脏、肝脏和肌肉中可以检测到[1-(13)C]丙酮酸盐及其代谢产物的超极化。在这项工作中，研究了在注射超极化[1-(13)C]丙酮酸盐和[2-(13)C]丙酮酸盐后，在体内测量大鼠脑中超极化[13C]代谢产物 13C 信号的可行性。注射[2-(13)C]丙酮酸盐导致检测到[2-(13)C]乳酸，但未检测到其他下游代谢物，如 TCA 循环中间产物。注射[1-(13)C]丙酮酸盐可同时检测到[1-(13)C]乳酸和[13C]碳酸氢盐。使用代谢模型拟合了在[1-(13)C]丙酮酸盐输注期间获得的超极化 13C 时间曲线，并确定了 VPDH 和 VLDH 的值。
