University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
Talanta. 2010 Jun 30;82(1):422-5. doi: 10.1016/j.talanta.2010.04.026. Epub 2010 Apr 22.
In this paper a concept of very simple measurement system dedicated for simultaneous photometric and fluorometric detection is presented. Only three ordinary unmodified light emitting diodes (LEDs) can be applied in this analytical device: one of them is used in the conventional way as a source of nearly monochromatic light inducing fluorescence, whereas two others are applied as photometric and fluorometric detectors of light. In this study quinine is chosen as a model analyte. The reported device enables simultaneous detection of this analyte in the micromolar range of concentration. The practical utility of prototype dual detector for complex sample analysis is illustrated by its application for determination of quinine in tonic water samples.
本文提出了一种非常简单的测量系统的概念，该系统专门用于同时进行光度法和荧光光度法检测。在这个分析设备中，仅使用三个普通的未经修饰的发光二极管（LED）：其中一个以常规方式用作近单色光的光源，激发荧光，而另外两个则用作光的光度法和荧光光度法检测器。在这项研究中，奎宁被选为模型分析物。所报道的设备能够在微摩尔浓度范围内同时检测该分析物。通过将原型双通道检测器应用于滋补水样品中奎宁的测定，说明了其在复杂样品分析中的实际应用。
