Fiedoruk-Pogrebniak Marta, Granica Mateusz, Koncki Robert
University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
Talanta. 2018 Feb 1;178:31-36. doi: 10.1016/j.talanta.2017.08.091. Epub 2017 Aug 31.
The utility of strikingly simple and cheap optoelectronic detectors operating according to a Paired-Emitter-Detector-Diode (PEDD) principle for microfluidic paper-based analytical devices (μPAD) has been experimentally confirmed. The prototypes of these compact detectors have been made of only two customary light emitting diodes without any additional optical parts like lens, filters, fibers etc. Moreover, for their operation economic and portable equipment is required (low-power circuit and ordinary voltmeter). Photometric and fluorometric PEDDs for paper devices are developed. In both cases the possibility of their applicability in transmittance and reflectance modes of measurements are presented. In these investigations as model analytes some coloured and fluorescence substances have been handled, but the utility of several developed systems for real chemical analysis has also been demonstrated. The results of photometric determination of hemoglobin in human blood, as well as fluorometric determination of quinine in tonics and calcium ions in mineral waters using various PEDD-μPAD systems have been shown.
根据配对发射极 - 探测器 - 二极管（PEDD）原理运行的极其简单且廉价的光电探测器在基于微流控纸的分析装置（μPAD）中的实用性已通过实验得到证实。这些紧凑型探测器的原型仅由两个常规发光二极管制成，无需任何额外的光学部件，如透镜、滤光片、光纤等。此外，其运行需要经济且便携的设备（低功率电路和普通电压表）。已开发出用于纸质装置的光度和荧光PEDD。在这两种情况下，都展示了它们在透射和反射测量模式下的适用性。在这些研究中，一些有色和荧光物质被用作模型分析物，但也证明了几种已开发系统在实际化学分析中的实用性。展示了使用各种PEDD - μPAD系统对人血中血红蛋白进行光度测定以及对滋补品中的奎宁和矿泉水中的钙离子进行荧光测定的结果。
