是否需要重复聚合酶链反应（PCR）来诊断艰难梭菌感染？
Is repeat PCR needed for diagnosis of Clostridium difficile infection?
机构信息
Stanford University School of Medicine, Department of Pathology, Palo Alto, CA 94304, USA.
出版信息
J Clin Microbiol. 2010 Oct;48(10):3738-41. doi: 10.1128/JCM.00722-10. Epub 2010 Aug 4.
Abstract
Patients with diarrhea, defined as loose or watery stool, and two or more Clostridium difficile tcdB PCR tests within 14 days of each other were investigated. Repeat PCR for 293 patients with a prior negative result yielded negative results in 396 (97.5%) of 406 tests. Ten new positives were detected, including one false positive. Repeat PCR within 7 days appears rarely useful, except for patients with evidence of a new infection.
摘要
对腹泻患者（定义为大便稀溏或水样便）和在相隔 14 天内进行了两次或以上艰难梭菌 tcdB PCR 检测的患者进行了调查。对 293 例先前检测结果为阴性的患者进行了 293 次重复 PCR 检测，其中 406 次检测结果为阴性（97.5%）。在这 10 例新的阳性检测中，包括 1 例假阳性。除了有新感染证据的患者外，在 7 天内进行重复 PCR 检测似乎很少有用。
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