用于金属增强荧光的粗糙银电极。
Roughened silver electrodes for use in metal-enhanced fluorescence.
作者信息
Geddes Chris D, Parfenov Alexandr, Roll David, Gryczynski Ignacy, Malicka Joanna, Lakowicz Joseph R
机构信息
Center for Fluorescence Spectroscopy, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, MD 21201, USA.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2004 Jul;60(8-9):1977-83. doi: 10.1016/j.saa.2003.10.014.
Roughened silver electrodes are widely used for surface-enhanced Raman scattering (SERS). We tested roughened silver electrodes for metal-enhanced fluorescence. Constant current between two silver electrodes in pure water resulted in the growth of fractal-like structures on the cathode. This electrode was coated with a monolayer of human serum albumin (HSA) protein that had been labeled with a fluorescent dye, indocyanine green (ICG). The fluorescence intensity of ICG-HSA on the roughened electrode increased by approximately 50-fold relative to the unroughened electrode, which was essentially non-fluorescent and increased typically two-fold as compared to the silver anode. No fractal-like structures were observed on the anode. Lifetime measurements showed that at least part of the increased intensity was due to an increased radiative decay rate of ICG. In our opinion, the use of in situ generated roughened silver electrodes will find multifarious applications in analytical chemistry, such as in fluorescence based assays, in an analogous manner to the now widespread use of SERS. To the best of our knowledge this is the first report of roughened silver electrodes for metal-enhanced fluorescence.
粗糙化银电极被广泛用于表面增强拉曼散射（SERS）。我们测试了粗糙化银电极用于金属增强荧光的情况。在纯水中，两个银电极之间的恒定电流导致阴极上生长出类似分形的结构。该电极涂覆有单层已用荧光染料吲哚菁绿（ICG）标记的人血清白蛋白（HSA）蛋白。与基本上无荧光且与银阳极相比通常增加两倍的未粗糙化电极相比，粗糙化电极上ICG-HSA的荧光强度增加了约50倍。阳极上未观察到类似分形的结构。寿命测量表明，强度增加的至少一部分是由于ICG的辐射衰减率增加。我们认为，原位生成的粗糙化银电极的使用将在分析化学中找到多种应用，例如在基于荧光的分析中，类似于现在广泛使用的SERS。据我们所知，这是关于粗糙化银电极用于金属增强荧光的首次报道。
Zotero 插件