Guerra Nelson Pérez, Fajardo Paula, Fuciños Clara, Amado Isabel Rodríguez, Alonso Elisa, Torrado Ana, Pastrana Lorenzo
Department of Analytical and Food Chemistry, Food Science and Technology Faculty, Ourense Campus, University of Vigo, 32004 Ourense, Spain.
J Biomed Biotechnol. 2010;2010. doi: 10.1155/2010/290286. Epub 2010 Jun 29.
The influence of initial pH on growth and nutrient (total sugars, nitrogen, and phosphorous) consumption by Enterococcus faecium CECT 410 was studied during batch cultures in whey. With these data, two realkalized fed-batch fermentations were developed using different feeding substrates. The shift from homolactic to mixed acid fermentation, the biphasic kinetics observed for cell growth and nitrogen consumption and the increase in the concentrations of biomass and products (lactic acid, acetic acid, ethanol, and butane-2,3-diol) were the most noteworthy observations of these cultures. Modelling the fed-batch growth of Ent. faecium with the Logistic and bi-Logistic models was not satisfactory. However, biomass production was best mathematically described with the use of a double Monod model, which was expressed in terms of biomass, product accumulation, and nitrogen utilization. Product formation was successfully modelled with a modified form of the Luedeking and Piret model developed in this study.
研究了初始pH值对粪肠球菌CECT 410在乳清分批培养过程中生长及营养物质（总糖、氮和磷）消耗的影响。利用这些数据，采用不同的进料底物进行了两次再碱化补料分批发酵。从同型乳酸发酵向混合酸发酵的转变、细胞生长和氮消耗观察到的双相动力学以及生物量和产物（乳酸、乙酸、乙醇和2,3-丁二醇）浓度的增加是这些培养中最值得注意的观察结果。用逻辑模型和双逻辑模型对粪肠球菌补料分批生长进行建模并不理想。然而，生物量生产用双莫诺德模型进行数学描述效果最佳，该模型以生物量、产物积累和氮利用来表示。产物形成用本研究开发的Luedeking和Piret模型的修正形式成功建模。
