Instituto de Biología y Genética Molecular, Valladolid, Spain.
Mediators Inflamm. 2010;2010. doi: 10.1155/2010/201929. Epub 2010 Jun 15.
The variable array of pattern receptor expression in different cells of the innate immune system explains the induction of distinct patterns of arachidonic acid (AA) metabolism. Peptidoglycan and mannan were strong stimuli in neutrophils, whereas the fungal extract zymosan was the most potent stimulus in monocyte-derived dendritic cells since it induced the production of PGE(2), PGD(2), and several cytokines including a robust IL-10 response. Zymosan activated kappaB-binding activity, but inhibition of NF-kappaB was associated with enhanced IL-10 production. In contrast, treatments acting on CREB (CRE binding protein), including PGE(2), showed a direct correlation between CREB activation and IL-10 production. Therefore, in dendritic cells zymosan induces il10 transcription by a CRE-dependent mechanism that involves autocrine secretion of PGE(2), thus unraveling a functional cooperation between eicosanoid production and cytokine production.
先天免疫系统不同细胞中模式受体表达的可变性解释了花生四烯酸 (AA) 代谢的不同模式的诱导。肽聚糖和甘露聚糖在中性粒细胞中是强烈的刺激物，而真菌提取物酵母聚糖在单核细胞衍生的树突状细胞中是最有效的刺激物，因为它诱导 PGE(2)、PGD(2) 和几种细胞因子的产生，包括强烈的 IL-10 反应。酵母聚糖激活 κB 结合活性，但 NF-κB 的抑制与增强的 IL-10 产生相关。相比之下，作用于 CREB（CRE 结合蛋白）的治疗方法，包括 PGE(2)，在 CREB 激活和 IL-10 产生之间显示出直接相关性。因此，在树突状细胞中，酵母聚糖通过涉及 PGE(2)自分泌的 CRE 依赖性机制诱导 il10 转录，从而揭示了类二十烷酸产生和细胞因子产生之间的功能协作。
