Departamento de Biotecnología, ETS Ing. Agrónomos, UPM, Madrid 28040, Spain.
Plant Cell Rep. 2010 Oct;29(10):1169-78. doi: 10.1007/s00299-010-0903-z. Epub 2010 Aug 6.
Changes in ascorbate peroxidase (APX) enzyme activity in response to nematode (Heterodera avenae) attack were studied in roots of three hexaploid wheat lines carrying Cre2, Cre5, or Cre7 nematode resistance genes and the susceptible Triticum aestivum cv. Anza. A spectrophotometric analysis was carried out with root extracts of infected plants 4, 7, 11, and 14 days after nematode inoculation using uninfected plant as control. APX induction in infected resistant genotypes was similar and higher than in the susceptible control. The introgression wheat/Aegilops ventricosa H-93-8 line, carrying the Cre2 gene, and its parental line H-10-15 as susceptible control were used to analyze whether this increase of activity was correlated with the induction of APX gene expression. Genes encoding cytosolic forms of APX were induced in roots of both lines in response to nematode infection. This induction took place both earlier and with greater intensity in the resistant line than in the susceptible one, and it was also higher in the root area at the site of nematode attachment.
研究了携带 Cre2、Cre5 或 Cre7 抗线虫基因的三个六倍体小麦品系以及易感的 Triticum aestivum cv. Anza 在受到线虫（ Heterodera avenae ）攻击时抗坏血酸过氧化物酶（APX）酶活性的变化。在接种线虫后 4、7、11 和 14 天，用未感染植物作为对照，对感染植物的根提取物进行分光光度分析。结果表明，在感染的抗性基因型中，APX 的诱导与易感对照相似且更高。携带 Cre2 基因的小麦/粗山羊草 H-93-8 系及其易感对照亲本 H-10-15 系被用来分析这种活性的增加是否与 APX 基因表达的诱导有关。在这两个系的根中，细胞溶质形式的 APX 基因在受到线虫感染时被诱导。这种诱导在抗性系中比在易感系中发生得更早且强度更大，并且在线虫附着部位的根区也更高。
