Mesa-Tejada R, Keydar I, Ramanarayanan M, Ohno T, Fenoglio C, Spiegelman S
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1529-33. doi: 10.1073/pnas.75.3.1529.
An antigen immunologically related to a group-specific antigen (gp52, a 52,000-dalton glycoprotein) of the mouse mammary tumor virus has been identified in paraffin sections of human breast cancers by means of the indirect immunoperoxidase technique. The specificity of the reaction with antibody against mouse mammary tumor virus was examined by absorption of the IgG with the following: (a) purified gp52; (b) a number of virus preparations (mouse mammary tumor virus, Rauscher leukemia virus, simian sarcoma virus, baboon endogenous virus, and Mason-Pfizer monkey virus); (c) normal plasma, leukocytes, breast tissue, milk, actin, collagen, and hyaluronic acid, all of human origin; (d) sheep erythrocytes and mucin. Only mouse mammary tumor virus (from C(3)H or Paris RIII strains and grown in either murine or feline cells) and purified gp52 eliminated the immunohistochemical reaction in the human breast tumors. Positive reactions were seen in 51 of 131 (39%) breast carcinomas of various histologic types, a minimal estimate in view of the limited number of sections from each tumor that could be examined. Negative reactions were obtained in all 119 benign breast lesions (cystic disease, fibroadenoma, papilloma, gynecomastia) and in all 18 normal breast tissues. With one exception, 99 carcinomas from 13 organs other than breast and 8 cystosarcomas were all negative.
通过间接免疫过氧化物酶技术，在人乳腺癌石蜡切片中鉴定出一种与小鼠乳腺肿瘤病毒的群特异性抗原（gp52，一种52,000道尔顿的糖蛋白）具有免疫相关性的抗原。用以下物质吸收IgG来检测与抗小鼠乳腺肿瘤病毒抗体反应的特异性：（a）纯化的gp52；（b）多种病毒制剂（小鼠乳腺肿瘤病毒、劳氏肉瘤病毒、猿猴肉瘤病毒、狒狒内源性病毒和梅森- Pfizer猴病毒）；（c）均来源于人的正常血浆、白细胞、乳腺组织、乳汁、肌动蛋白、胶原蛋白和透明质酸；（d）绵羊红细胞和粘蛋白。只有小鼠乳腺肿瘤病毒（来自C(3)H或巴黎RIII株，在鼠细胞或猫细胞中培养）和纯化的gp52消除了人乳腺肿瘤中的免疫组织化学反应。在131例不同组织学类型的乳腺癌中有51例（39%）出现阳性反应，鉴于每个肿瘤可检查的切片数量有限，这只是一个最低估计值。在所有119例良性乳腺病变（囊性疾病、纤维腺瘤、乳头状瘤、男性乳房发育症）和所有18例正常乳腺组织中均获得阴性反应。除1例例外，来自乳腺以外13个器官的99例癌和8例囊肉瘤均为阴性。
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