Mesa-Tejada R, Keydar I, Ramanarayanan M, Ohno T, Fenoglio C, Spiegelman S
Ann Clin Lab Sci. 1979 May-Jun;9(3):202-11.
The localization of antigenic components with cross-reactivity to a 52,000 dalton group specific glycoprotein (gp52) of the mouse mammary tumor virus (MMTV) in paraffin sections of human breast carcinomas is described using an indirect immunoperoxidase method. This method was first optimized on paraffin sections of mouse mammary tumors. The specificity of the reaction observed in the human tissues was established by absorption of the specific IgG with: a) purified gp52; b) several relevant and irrelevant viral preparations; c) normal human plasma, leukocytes, breast tissue, milk, actin, collagen, and hyaluronic acid; d) sheep erythrocytes, bovine mucin and fetal calf serum. Only MMTV and purified gp52 eliminated the immunohistochemical reaction in human breast tumors. Positive reactions were seen in 171 of 376 (45.5 percent) randomly selected breast carcinomas of various histopathologic types, while negative reactions were obtained in all 137 normal and benign cases tested. In those invasive tumors with an intraductal component, a higher percentage (63.9 percent) of positive cases was seen. A positive reaction of different specificity was observed in foci of apocrine metaplasia. With one exception, 99 carcinomas from 13 organs other than breast and eight cystosarcomas were negative.
使用间接免疫过氧化物酶法描述了在人乳腺癌石蜡切片中与小鼠乳腺肿瘤病毒（MMTV）的52,000道尔顿组特异性糖蛋白（gp52）具有交叉反应性的抗原成分的定位。该方法首先在小鼠乳腺肿瘤石蜡切片上进行了优化。通过用以下物质吸收特异性IgG来确定在人体组织中观察到的反应的特异性：a）纯化的gp52；b）几种相关和不相关的病毒制剂；c）正常人血浆、白细胞、乳腺组织、乳汁、肌动蛋白、胶原蛋白和透明质酸；d）绵羊红细胞、牛粘蛋白和胎牛血清。只有MMTV和纯化的gp52消除了人乳腺肿瘤中的免疫组织化学反应。在随机选择的376例各种组织病理学类型的乳腺癌中，有171例（45.5%）呈阳性反应，而在所有137例检测的正常和良性病例中均为阴性反应。在那些具有导管内成分的浸润性肿瘤中，阳性病例的百分比更高（63.9%）。在大汗腺化生灶中观察到不同特异性的阳性反应。除1例例外，来自乳腺以外13个器官的99例癌和8例囊肉瘤均为阴性。
