Département d'Anatomie et Cytologie Pathologiques, CHU Pontchaillou, 2 rue Henri Le Guilloux, 35033 Rennes Cedex 09, France.
J Neurosci Methods. 2010 Sep 30;192(1):102-9. doi: 10.1016/j.jneumeth.2010.07.041. Epub 2010 Aug 6.
We used high-magnetic field (4.7 T) magnetic resonance imaging (MRI) to build the first high-resolution (100 microm x 150 microm x 100 microm) three-dimensional (3D) digital atlas in stereotaxic coordinates of the brain of a female domestic pig (Sus scrofa domesticus). This atlas was constructed from one hemisphere which underwent a symmetrical transformation through the midsagittal plane. Concomitant construction of a 3D histological atlas based on the same scheme facilitated control of deep brain structure delimitation and enabled cortical mapping to be achieved. The atlas contains 178 individual cerebral structures including 42 paired and 9 single deep brain structures, 5 ventricular system areas, 6 paired deep cerebellar nuclei, 12 cerebellar lobules and 28 cortical areas per hemisphere. Given the increasing importance of pig brains in medical research, this atlas should be a useful tool for intersubject normalization in anatomical imaging as well as for precisely localizing brain areas in functional MR studies or electrode implantation trials. The atlas can be freely downloaded from our institution's Website.
我们使用高磁场（4.7T）磁共振成像（MRI）构建了第一个女性家猪（Sus scrofa domesticus）大脑的立体定向坐标高分辨率（100 微米 x 150 微米 x 100 微米）三维（3D）数字图谱。该图谱由通过正中矢状面进行对称变换的一个脑半球构建而成。同时基于相同方案构建的 3D 组织学图谱有助于控制深部脑结构的界定，并实现皮质映射。图谱包含 178 个单独的脑结构，包括 42 对和 9 个单独的深部脑结构、5 个脑室系统区域、6 对深部小脑核、12 个小脑叶和每侧半球的 28 个皮质区域。鉴于猪脑在医学研究中的重要性日益增加，该图谱应该成为解剖成像中受试者间标准化以及在功能磁共振研究或电极植入试验中精确定位脑区的有用工具。图谱可以从我们机构的网站上免费下载。
