Sensory Neurobiology Group, School of Biomedical Sciences, University of Queensland, St Lucia Qld 4072, Australia.
Neuroimage. 2010 May 15;51(1):76-82. doi: 10.1016/j.neuroimage.2010.01.086. Epub 2010 Feb 6.
In the past three decades, the zebrafish has become a vital animal model in a range of biological sciences. To augment current neurobiological research, we have developed the first three-dimensional digital atlas of the zebrafish brain from T2-weighted magnetic resonance histology (MRH) images acquired on a 16.4-T superconducting magnet. We achieved an isotropic resolution of 10 microm, which is the highest resolution achieved in a vertebrate brain and, for the first time, is comparable in slice thickness to conventional histology. By using manual segmentation, 53 anatomical structures, including fiber tracts as small as 40 microm, were delineated. Using Amira software, structures were also individually segmented and reconstructed to create three-dimensional animations. Additional quantitative information including, volume, surface areas, and mean gray scale intensities were also determined. Finally, we established a stereotaxic coordinate system as a framework in which maps created from other modalities can be incorporated into the atlas.
在过去的三十年中，斑马鱼已成为多个生物学领域的重要动物模型。为了增强当前的神经生物学研究，我们利用在 16.4T 超导磁体上采集的 T2 加权磁共振组织学（MRH）图像，开发了第一个斑马鱼大脑的三维数字图谱。我们实现了 10 微米的各向同性分辨率，这是在脊椎动物大脑中实现的最高分辨率，并且首次与传统组织学切片的厚度相当。通过手动分割，我们描绘了包括直径小至 40 微米的纤维束在内的 53 个解剖结构。使用 Amira 软件，我们还对每个结构进行了单独分割和重建，以创建三维动画。此外，还确定了包括体积、表面积和平均灰度强度在内的其他定量信息。最后，我们建立了一个立体定向坐标系，作为一个框架，可以将来自其他模式的图谱整合到图谱中。
