Marinovich M, Viviani B, Galli C L
Laboratory of Toxicology, Institute of Pharmacological Sciences, University of Milan, via Balzaretti 9, I-20133 Milan, Italy.
Toxicol In Vitro. 1994 Feb;8(1):91-8. doi: 10.1016/0887-2333(94)90212-7.
20 shampoos and their component surfactants were studied in vitro using a murine epidermal cell line (HEL/30). Lactate dehydrogenase (LDH) leakage into the cell medium and the inhibition of protein neosynthesis were evaluated as markers of cytotoxicity after 2 hr of exposure. The coefficients of correlation were -0.83 and -0.69 for comparisons between total surfactant content and inhibition of protein synthesis and leakage of LDH, respectively. There was an inverse relationship between the inhibition of protein synthesis and the amphoteric surfactant content of the shampoo. An excellent correlation (r = 0.94) was found between LDH leakage values and scores from eye irritation tests in vivo on seven of the shampoos. The results obtained suggest that this model allows quantitative evaluation of the cytotoxicity of detergent products (shampoos) and of the contribution of their major components (surfactants) to that toxicity.
使用小鼠表皮细胞系（HEL/30）对20种洗发水及其成分表面活性剂进行了体外研究。在暴露2小时后，评估乳酸脱氢酶（LDH）泄漏到细胞培养基中以及蛋白质新合成的抑制情况，以此作为细胞毒性的标志物。总表面活性剂含量与蛋白质合成抑制以及LDH泄漏之间比较的相关系数分别为-0.83和-0.69。洗发水的蛋白质合成抑制与两性表面活性剂含量之间存在负相关关系。在七种洗发水中，发现LDH泄漏值与体内眼刺激试验得分之间具有良好的相关性（r = 0.94）。所得结果表明，该模型能够对洗涤剂产品（洗发水）的细胞毒性及其主要成分（表面活性剂）对该毒性的贡献进行定量评估。
