North-Root H, Yackovich F, Demetrulias J, Gacula M, Heinze J E
Food Chem Toxicol. 1985 Feb;23(2):271-3. doi: 10.1016/0278-6915(85)90028-6.
Shampoos predicted to vary over a wide range in their potential to cause eye irritancy were tested in SIRC rabbit corneal cells for cytotoxicity. Six shampoos at at least ten different concentrations were added to culture dishes containing the SIRC cells and were administered at a dose of 0.01 ml directly to the corneal surface of the albino rabbit eye. When the shampoos were ranked both according to their relative toxicities in vitro and according to their relative irritancies in the modified Draize test the resulting rank orders were in perfect agreement. The results demonstrate that the SIRC cell line is a suitable model system for screening shampoos of differing surfactant compositions without the need to use live animals. Further, results obtained from the SIRC cell assay should aid in the selection of final product formulations for in vivo testing as part of the pre-market clearance process.
对预计在引起眼睛刺激的可能性方面有很大差异的洗发水进行了SIRC兔角膜细胞的细胞毒性测试。将六种洗发水以至少十种不同浓度添加到含有SIRC细胞的培养皿中，并以0.01毫升的剂量直接施用于白化兔眼的角膜表面。当根据洗发水在体外的相对毒性以及在改良的Draize试验中的相对刺激性进行排名时，所得的排名顺序完全一致。结果表明，SIRC细胞系是一种合适的模型系统，用于筛选具有不同表面活性剂组成的洗发水，而无需使用活体动物。此外，从SIRC细胞试验获得的结果应有助于选择最终产品配方进行体内测试，作为上市前批准过程的一部分。
