Merrick P, Nieminen A L, Harper R A, Herman B, Lemasters J J
Laboratories for Cell Biology, Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill, NC 27599-7090, USA.
Toxicol In Vitro. 1992 Nov;6(6):543-7. doi: 10.1016/0887-2333(92)90066-z.
The irritancy potential of seven shampoos was evaluated by a rapid cytotoxicity assay in cultured human keratinocytes and rat hepatocytes. Loss of cell viability was estimated from increases in propidium iodide fluorescence measured using a multiwell fluorescence scanner. The concentration of shampoo causing a 50% loss of cell viability after 15 min of incubation (V(50)) was determined by probit analysis. Log V(50) measured in human keratinocytes showed a strong negative correlation (r = -0.95; P <0.001) with Draize eye scores in rabbits. Log V(50) measured in rat hepatocytes did not show a statistically significant correlation with Draize eye scores. The results indicate that cytotoxicity screening of human keratinocytes using propidium iodide and a multiwell fluorescence scanner is highly predictive of Draize eye scores for surfactant-containing shampoos.
通过在培养的人角质形成细胞和大鼠肝细胞中进行快速细胞毒性试验，评估了七种洗发水的刺激潜力。使用多孔荧光扫描仪测量碘化丙啶荧光的增加来估计细胞活力的丧失。通过概率分析确定孵育15分钟后导致细胞活力丧失50%的洗发水浓度（V(50)）。在人角质形成细胞中测得的Log V(50)与兔眼Draize评分呈强负相关（r = -0.95；P <0.001）。在大鼠肝细胞中测得的Log V(50)与兔眼Draize评分未显示出统计学上的显著相关性。结果表明，使用碘化丙啶和多孔荧光扫描仪对人角质形成细胞进行细胞毒性筛选，对于含表面活性剂洗发水的兔眼Draize评分具有高度预测性。
