Roguet R, Régnier M, Cohen C, Dossou K G, Rougier A
Laboratoires de Recherche de l'Oréal, 1 av. E. Schueller 93600, Aulnay-sous-bois, France.
Toxicol In Vitro. 1994 Aug;8(4):635-9. doi: 10.1016/0887-2333(94)90033-7.
As they simulate the multilayer tissue architecture present in vivo, three-dimensional (3D) cultures of skin epithelial cells represent an attractive alternative for in vitro irritancy testing. In this study histology of non-commercial (Skin recombinant on de-epidermized dermis) and commercial models (Episkin, ZK 1300, Living Skin Equivalent) was compared. All 3D models possess a stratified epidermis with a differentiated stratum corneum. Their barrier function has been demonstrated by comparison of surfactants cytotoxicity on the 3D models and on human keratinocyte monolayer culture, and by the evaluation of the tritiated water penetration. All the 3D models showed permeability higher than fresh skin, the skin recombinant on DDED being the less permeable. However, reconstituted skin models possess several advantages for dermotoxicity testing, including the standardized nature of the epithelia and their suitability for testing water-insoluble compounds.
由于它们模拟了体内存在的多层组织结构，皮肤上皮细胞的三维（3D）培养代表了一种用于体外刺激性测试的有吸引力的替代方法。在本研究中，比较了非商业模型（脱表皮真皮上的皮肤重组体）和商业模型（Episkin、ZK 1300、活皮肤等效物）的组织学。所有3D模型都具有分层的表皮和分化的角质层。通过比较表面活性剂对3D模型和人角质形成细胞单层培养物的细胞毒性，以及通过评估氚标记水的渗透性，证明了它们的屏障功能。所有3D模型的渗透性均高于新鲜皮肤，脱表皮真皮上的皮肤重组体渗透性最低。然而，重组皮肤模型在皮肤毒性测试方面具有几个优点，包括上皮细胞的标准化性质及其对测试水不溶性化合物的适用性。
