Regnier M, Caron D, Reichert U, Schaefer H
Centre International de Recherches Dermatologiques Galderma, Sophia Antipolis, Valbonne, France.
J Pharm Sci. 1993 Apr;82(4):404-7. doi: 10.1002/jps.2600820414.
The present study compares the stratum corneum of human skin and human reconstructed epidermis by histological examination as well as by estimation of water permeability. Measurement of the percutaneous absorption of benzoic acid, testosterone, and hydrocortisone revealed that under the same experimental conditions the reconstructed epidermis on de-epidermized dermis exhibits a barrier function with qualitative properties similar to that of normal skin: water and benzoic acid penetrate more rapidly than testosterone and hydrocortisone. Quantitatively, however, reconstructed epidermis is more permeable than normal human skin. This points to an impaired barrier function of the epidermis reconstructed in vitro.
本研究通过组织学检查以及水渗透性评估，比较了人类皮肤角质层与人类重建表皮。苯甲酸、睾酮和氢化可的松经皮吸收的测量结果显示，在相同实验条件下，脱表皮真皮上的重建表皮具有与正常皮肤类似的屏障功能：水和苯甲酸的渗透速度比睾酮和氢化可的松更快。然而，从定量角度来看，重建表皮比正常人类皮肤的渗透性更高。这表明体外重建的表皮屏障功能受损。
