Seishima M, Kudo Y, Nagao S, Mori S, Nozawa Y
Department of Dermatology, Gifu University School of Medicine, Japan.
Arch Dermatol Res. 1991;283(2):96-9. doi: 10.1007/BF00371616.
Intracellular Ca2+ concentrations ([Ca2+]i) in cultured skin fibroblasts from normal subjects and progressive systemic sclerosis (PSS) patients were determined by using Fura-2 and fluorescent videomicroscopy. With the exception of fibroblasts from one PSS patient showing a higher [Ca2+]i, no significant difference was observed in resting [Ca2+]i between the two groups of fibroblasts. Bradykinin (BK) (10 microM) induced a transient [Ca2+]i increase in normal fibroblasts, whereas the BK-induced [Ca2+]i increase was reduced or not detectable in PSS fibroblasts. Removal of extracellular Ca2+ did not eliminate the BK-induced [Ca2+]i increase in normal fibroblasts. These findings suggest that BK stimulates Ca2+ release from intracellular stores in human fibroblasts, and also that the BK-mediated Ca2+ release is impaired in PSS fibroblasts.
采用Fura - 2和荧光视频显微镜测定了正常受试者和进行性系统性硬化症（PSS）患者培养的皮肤成纤维细胞内的钙离子浓度（[Ca2+]i）。除了一名PSS患者的成纤维细胞显示出较高的[Ca2+]i外，两组成纤维细胞的静息[Ca2+]i未观察到显著差异。缓激肽（BK）（10微摩尔）可诱导正常成纤维细胞内[Ca2+]i短暂升高，而在PSS成纤维细胞中，BK诱导的[Ca2+]i升高减少或未检测到。去除细胞外Ca2+并不能消除BK诱导的正常成纤维细胞内[Ca2+]i升高。这些发现表明，BK刺激人成纤维细胞内储存的Ca2+释放，并且BK介导的Ca2+释放在PSS成纤维细胞中受损。
