Barua Chandana Choudhury, Buragohain Lipika, Rizavi Hooriyah, Gogoi Samir Bikash, Rahman Farida, Siva Bandi, Mounika K, Babu K Suresh, Chandra Pathak Debesh, Phukan Arabinda
Department of Pharmacology and Toxicology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam, 781022, India.
Department of Pharmacology and Toxicology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam, 781022, India.
J Ayurveda Integr Med. 2020 Oct-Dec;11(4):464-470. doi: 10.1016/j.jaim.2018.02.140. Epub 2019 Jan 8.
Entada phaseoloides is a well-known medicinal plant traditionally used in Ayurvedic medicine for centuries.
To evaluate the anti-stress activity of seeds of E. phaseoloides in endoplasmic reticulum stress during chronic restrain stress in mice, based on our preliminary screening.
Mice (n = 6/group) were restrained daily for 6 h in 50 ml polystyrene tubes for 28 days. Methanolic extract of E. phaseoloides (MEEP) (100 and 200 mg/kg, p.o.) and standard drug, imipramine (10 mg/kg i.p.) were administered daily 45 min prior to restrain from day 22-28. Then, forced swim test (FST) was performed to assess despair behavior. Lipid peroxidation (LPO) and antioxidant enzymes Reduced glutathione (GSH), Superoxide dismutase (SOD) were measured in the hippocampus of mice. 78 kDa Glucose-regulated Protein, 94 kDa Glucose-regulated Protein, C/EBP homologous protein, Caspase-12 expression were quantified by Real Time PCR.
MEEP significantly reduced the immobility time in FST (P < 0.001). Significant reduction of LPO (P < 0.05) level and restored antioxidant enzymes viz. GSH (P < 0.001) and SOD towards vehicle control group were observed. Down-regulation of genes GRP 78, GRP 94 (P < 0.001), CHOP and Caspase-12 (P < 0.001) as compared to the chronic restrain stress group was evident, which were upregulated following treatment. Isolation of the active components of the seeds revealed the presence of Oleic acid (1), Entadamide A (2), Entadamide A-beta-d-glucopyranoside (3) and 1-O-protocatechuoyl-β-d-glucose.
MEEP altered endoplasmic reticulum stress in chronic restrain stressed mice; however, as an antidepressant it showed a weaker response.
榼藤子是一种著名的药用植物，在阿育吠陀医学中已被传统使用了几个世纪。
基于我们的初步筛选，评估榼藤子种子在小鼠慢性束缚应激期间对内质网应激的抗应激活性。
将小鼠（每组n = 6）每天在50 ml聚苯乙烯管中束缚6小时，持续28天。从第22天至28天，每天在束缚前45分钟口服给予榼藤子甲醇提取物（MEEP）（100和200 mg/kg）和标准药物丙咪嗪（10 mg/kg，腹腔注射）。然后，进行强迫游泳试验（FST）以评估绝望行为。测定小鼠海马中的脂质过氧化（LPO）和抗氧化酶还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）。通过实时PCR定量78 kDa葡萄糖调节蛋白、94 kDa葡萄糖调节蛋白、C/EBP同源蛋白、半胱天冬酶-12的表达。
MEEP显著减少了FST中的不动时间（P < 0.001）。观察到LPO水平显著降低（P < 0.05），抗氧化酶即GSH（P < 0.001）和SOD恢复至溶剂对照组水平。与慢性束缚应激组相比，基因GRP 78、GRP 94（P < 0.001）、CHOP和半胱天冬酶-12（P < 0.001）明显下调，治疗后这些基因上调。种子活性成分的分离显示存在油酸（1）、榼藤酰胺A（2）、榼藤酰胺A-β-D-吡喃葡萄糖苷（3）和1-O-原儿茶酰基-β-D-葡萄糖。
MEEP改变了慢性束缚应激小鼠的内质网应激；然而，作为一种抗抑郁药，它的反应较弱。
