URMITE CNRS-IRD UMR6236, IFR 48, Faculté de Médecine et de Pharmacie, Marseille, France.
FEMS Microbiol Lett. 2010 Sep 1;310(2):158-67. doi: 10.1111/j.1574-6968.2010.02058.x. Epub 2010 Jul 9.
Bartonella henselae is an emerging gram-negative facultative intracellular pathogen transmitted via Ctenocephalides felis (cat fleas) or cat scratches. Bartonellosis is present mainly in the form of cat scratch disease (CSD), bacillary angiomatosis and infective endocarditis (IE). The methods used to diagnose B. henselae rely on culturing, immunofluorescent assays and molecular techniques. The objective of the present study was to identify candidate proteins for the serodiagnosis of bartonellosis with the differential discrimination of both clinical scenarios: CSD and IE. For this, an immunoproteomic approach combined with 2-DE, immunoblotting and matrix-assisted laser desorption/ionization time-of-flight MS has been developed. Immunoproteomic profiles of sera collected from patients with CSD and IE were compared with those of blood donors. We identified several candidate proteins as phage-encoding Pap31 protein and an outer membrane protein of BH11510 that, in our view, might be useful for the serodiagnosis of bartonellosis.
汉赛巴尔通体是一种新兴的革兰氏阴性兼性细胞内病原体，通过猫栉首蚤（猫蚤）或猫抓伤传播。巴尔通体病主要表现为猫抓病（CSD）、杆菌性血管瘤病和感染性心内膜炎（IE）。用于诊断汉赛巴尔通体的方法依赖于培养、免疫荧光分析和分子技术。本研究的目的是鉴定候选蛋白，用于巴尔通体病的血清学诊断，同时区分两种临床情况：CSD 和 IE。为此，我们开发了一种免疫蛋白质组学方法，结合 2-DE、免疫印迹和基质辅助激光解吸/电离飞行时间 MS。比较了来自 CSD 和 IE 患者的血清与献血者的免疫蛋白质组图谱。我们鉴定了几种候选蛋白，如噬菌体编码的 Pap31 蛋白和 BH11510 的外膜蛋白，我们认为这些蛋白可能对巴尔通体病的血清学诊断有用。
