McCool Tera L, Hoey John G, Montileone Francesca, Goldenberg Hannah B, Mordechai Eli, Adelson Martin E
Medical Diagnostic Laboratories, Hamilton, NJ 08690-3303, USA.
Diagn Microbiol Infect Dis. 2008 Jan;60(1):17-23. doi: 10.1016/j.diagmicrobio.2007.07.017. Epub 2007 Sep 21.
The antibody response to Bartonella henselae has been studied in a number of mammals; however, the human response needs to be further studied. After natural infection, humans have antibody reactivity to a large number of B. henselae proteins. We used a proteomic approach to identify antigenic proteins of B. henselae to determine their capacity to elicit a human antibody response. Comparing patient sera by Western blot analysis demonstrated significant amounts of reactivity to B. henselae. The immunofluorescence assay (IFA)-positive sera identified several protein spots of interest. However, a consistent reactivity to a single spot by all sera was not observed. Three of these spots demonstrated reactivity in 71%, 64%, and 64% of positive sera tested with negligible reactivity to the negative sera. These proteins were identified as GroES, BepA, and GroEL. Most IFA-positive sera demonstrated reactivity to GroES, GroEL, and BepA. The usefulness of these proteins for a clinical serologic assay is discussed.
对多种哺乳动物体内针对汉赛巴尔通体的抗体反应进行了研究；然而，人类的反应仍需进一步研究。自然感染后，人类对大量汉赛巴尔通体蛋白具有抗体反应性。我们采用蛋白质组学方法鉴定汉赛巴尔通体的抗原蛋白，以确定它们引发人类抗体反应的能力。通过蛋白质印迹分析比较患者血清，结果显示对汉赛巴尔通体有显著的反应性。免疫荧光试验（IFA）阳性血清鉴定出了几个感兴趣的蛋白斑点。然而，并未观察到所有血清对单个斑点都有一致的反应性。在检测的阳性血清中，其中三个斑点的反应性分别为71%、64%和64%，而对阴性血清的反应性可忽略不计。这些蛋白被鉴定为GroES、BepA和GroEL。大多数IFA阳性血清对GroES、GroEL和BepA有反应性。文中讨论了这些蛋白在临床血清学检测中的实用性。
