Ryan U M, Samarasinghe B, Read C, Buddle J R, Robertson I D, Thompson R C A
Division of Health Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
Appl Environ Microbiol. 2003 Jul;69(7):3970-4. doi: 10.1128/AEM.69.7.3970-3974.2003.
Over a 3-year period, a total of 646 fecal samples from pigs in 22 indoor and outdoor herds from Western Australia were screened for Cryptosporidium spp. by microscopy. Results revealed that 39 of 646 samples (6.03%) were positive for Cryptosporidium. Cryptosporidium was much more common in outdoor herds (17.2%) than in indoor herds (0.5%) and was more common in animals between the ages of 5 and 8 weeks (69.2%) than in younger animals (P < 0.0001). Molecular characterization of the positive samples at the 18S ribosomal DNA locus identified two distinct genotypes of Cryptosporidium: the previously identified pig genotype I and a novel pig genotype (pig genotype II), both of which warrant species status.
在3年期间，通过显微镜检查对西澳大利亚22个室内和室外猪群的646份猪粪便样本进行了隐孢子虫属筛查。结果显示，646份样本中有39份（6.03%）隐孢子虫呈阳性。隐孢子虫在室外猪群（17.2%）中比在室内猪群（0.5%）中更为常见，并且在5至8周龄的动物（69.2%）中比在年幼动物中更为常见（P < 0.0001）。对18S核糖体DNA位点的阳性样本进行分子特征分析，鉴定出两种不同的隐孢子虫基因型：先前鉴定的猪基因型I和一种新的猪基因型（猪基因型II），这两种基因型都应具有物种地位。
