猪体内一种新型隐孢子虫基因型的鉴定。
Identification of a novel Cryptosporidium genotype in pigs.
作者信息
Ryan U M, Samarasinghe B, Read C, Buddle J R, Robertson I D, Thompson R C A
机构信息
Division of Health Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
出版信息
Appl Environ Microbiol. 2003 Jul;69(7):3970-4. doi: 10.1128/AEM.69.7.3970-3974.2003.
Abstract
Over a 3-year period, a total of 646 fecal samples from pigs in 22 indoor and outdoor herds from Western Australia were screened for Cryptosporidium spp. by microscopy. Results revealed that 39 of 646 samples (6.03%) were positive for Cryptosporidium. Cryptosporidium was much more common in outdoor herds (17.2%) than in indoor herds (0.5%) and was more common in animals between the ages of 5 and 8 weeks (69.2%) than in younger animals (P < 0.0001). Molecular characterization of the positive samples at the 18S ribosomal DNA locus identified two distinct genotypes of Cryptosporidium: the previously identified pig genotype I and a novel pig genotype (pig genotype II), both of which warrant species status.
摘要
在3年期间，通过显微镜检查对西澳大利亚22个室内和室外猪群的646份猪粪便样本进行了隐孢子虫属筛查。结果显示，646份样本中有39份（6.03%）隐孢子虫呈阳性。隐孢子虫在室外猪群（17.2%）中比在室内猪群（0.5%）中更为常见，并且在5至8周龄的动物（69.2%）中比在年幼动物中更为常见（P < 0.0001）。对18S核糖体DNA位点的阳性样本进行分子特征分析，鉴定出两种不同的隐孢子虫基因型：先前鉴定的猪基因型I和一种新的猪基因型（猪基因型II），这两种基因型都应具有物种地位。
相似文献
1
Identification of a novel Cryptosporidium genotype in pigs.猪体内一种新型隐孢子虫基因型的鉴定。
Appl Environ Microbiol. 2003 Jul;69(7):3970-4. doi: 10.1128/AEM.69.7.3970-3974.2003.
2
Prevalence of the Cryptosporidium pig genotype II in pigs from the Yangtze River Delta, China.中国长江三角洲猪群中Ⅱ型隐孢子虫的流行情况。
PLoS One. 2011;6(6):e20738. doi: 10.1371/journal.pone.0020738. Epub 2011 Jun 3.
3
Prevalence of Cryptosporidium genotypes in pre and post-weaned pigs in Australia.澳大利亚断奶前后仔猪隐孢子虫基因型的流行情况。
Exp Parasitol. 2008 Jul;119(3):418-21. doi: 10.1016/j.exppara.2008.04.009. Epub 2008 Apr 14.
4
Prevalence and molecular identification of Cryptosporidium spp. in pigs in Henan, China.中国河南猪源隐孢子虫的流行情况及分子鉴定。
Parasitol Res. 2010 Nov;107(6):1489-94. doi: 10.1007/s00436-010-2024-6. Epub 2010 Aug 10.
5
Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic.捷克共和国南波希米亚地区屠宰场猪源隐孢子虫分离株的分子特征分析。
Parasitol Res. 2009 Jan;104(2):425-8. doi: 10.1007/s00436-008-1215-x. Epub 2008 Oct 11.
6
Molecular characterization of Cryptosporidium in pigs in central Vietnam.越南北中部猪源隐孢子虫的分子特征。
Parasitol Res. 2013 Jan;112(1):187-92. doi: 10.1007/s00436-012-3124-2. Epub 2012 Oct 5.
7
Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark.丹麦猪和牛源隐孢子虫与贾第虫的分子及系统发育特征分析
Parasitology. 2007 Mar;134(Pt 3):339-50. doi: 10.1017/S0031182006001533. Epub 2006 Nov 1.
8
Cryptosporidium suis n. sp. (Apicomplexa: Cryptosporidiidae) in pigs (Sus scrofa).猪隐孢子虫新种（顶复门：隐孢子虫科）在猪（野猪）中的发现
J Parasitol. 2004 Aug;90(4):769-73. doi: 10.1645/GE-202R1.
9
Cryptosporidium suis infection in post-weaned and adult pigs in Shaanxi province, northwestern China.中国西北部陕西省断奶后仔猪和成年猪的猪隐孢子虫感染
Korean J Parasitol. 2015 Feb;53(1):113-7. doi: 10.3347/kjp.2015.53.1.113. Epub 2015 Feb 27.
10
Molecular characterization of Cryptosporidium isolates from pigs in Zaragoza (northeastern Spain).西班牙东北部萨拉戈萨猪源隐孢子虫分离株的分子特征分析
Vet Parasitol. 2007 Sep 30;148(3-4):231-5. doi: 10.1016/j.vetpar.2007.06.022. Epub 2007 Aug 1.
引用本文的文献
1
Occurrence and Molecular Characterization of spp. in Swine Farms in Northeastern Spain.西班牙东北部猪场中 spp. 的发生情况及分子特征分析
Pathogens. 2025 Jul 5;14(7):665. doi: 10.3390/pathogens14070665.
2
Global prevalence of spp. in pigs: a systematic review and meta-analysis.猪源 spp. 的全球流行率：系统评价和荟萃分析。
Parasitology. 2023 May;150(6):531-544. doi: 10.1017/S0031182023000276. Epub 2023 Mar 20.
3
Detection and molecular characterisation of Cryptosporidium spp. in Swedish pigs.瑞典猪中隐孢子虫属的检测和分子特征分析。
Acta Vet Scand. 2020 Jul 29;62(1):40. doi: 10.1186/s13028-020-00537-z.
4
Cryptosporidium infections in terrestrial ungulates with focus on livestock: a systematic review and meta-analysis.以家畜为重点的陆生有蹄类动物中隐孢子虫感染：系统评价和荟萃分析。
Parasit Vectors. 2019 Sep 14;12(1):453. doi: 10.1186/s13071-019-3704-4.
5
Cryptosporidium suis infection in post-weaned and adult pigs in Shaanxi province, northwestern China.中国西北部陕西省断奶后仔猪和成年猪的猪隐孢子虫感染
Korean J Parasitol. 2015 Feb;53(1):113-7. doi: 10.3347/kjp.2015.53.1.113. Epub 2015 Feb 27.
6
Age-related detection and molecular characterization of Cryptosporidium suis and Cryptosporidium scrofarum in pre- and post-weaned piglets and adult pigs in Japan.日本断奶前和断奶后仔猪及成年猪中猪隐孢子虫和猪微小隐孢子虫的年龄相关检测和分子特征。
Parasitol Res. 2014 Jan;113(1):359-65. doi: 10.1007/s00436-013-3662-2. Epub 2013 Nov 5.
7
Cryptosporidium suis and Cryptosporidium scrofarum in Eurasian wild boars (Sus scrofa) in Central Europe.中欧野猪（Sus scrofa）中猪隐孢子虫和猪隐孢子虫。
Vet Parasitol. 2013 Nov 8;197(3-4):504-8. doi: 10.1016/j.vetpar.2013.07.003. Epub 2013 Jul 12.
8
Prevalence and genetic characterizations of Cryptosporidium spp. in pre-weaned and post-weaned piglets in Heilongjiang Province, China.中国黑龙江省未断奶和断奶仔猪中隐孢子虫属的流行情况和遗传特征。
PLoS One. 2013 Jul 3;8(7):e67564. doi: 10.1371/journal.pone.0067564. Print 2013.
9
Occurrence of Cryptosporidium suis and Cryptosporidium scrofarum on commercial swine farms in the Czech Republic and its associations with age and husbandry practices.在捷克共和国的商业养猪场中猪隐孢子虫和猪小隐孢子虫的发生情况及其与年龄和饲养方式的关系。
Parasitol Res. 2013 Mar;112(3):1143-54. doi: 10.1007/s00436-012-3244-8. Epub 2012 Dec 28.
10
Molecular characterization of Cryptosporidium in pigs in central Vietnam.越南北中部猪源隐孢子虫的分子特征。
Parasitol Res. 2013 Jan;112(1):187-92. doi: 10.1007/s00436-012-3124-2. Epub 2012 Oct 5.
本文引用的文献
1
Pathogenicity of Cryptosporidium parvum--evaluation of an animal infection model.微小隐孢子虫的致病性——一种动物感染模型的评估
Vet Parasitol. 2003 Apr 2;113(1):35-57. doi: 10.1016/s0304-4017(03)00034-7.
2
Improved staining method for detecting Cryptosporidium oocysts in stools using malachite green.使用孔雀石绿检测粪便中隐孢子虫卵囊的改良染色方法。
J Gen Appl Microbiol. 1999 Jun;45(3):139-142. doi: 10.2323/jgam.45.139.
3
Prevalence of Cryptosporidium parvum infections in weaned piglets and fattening porkers in Kanagawa Prefecture, Japan.
Jpn J Infect Dis. 2001 Feb;54(1):23-6.
4
Prevalence of enteropathogens in suckling and weaned piglets with diarrhoea in southern Germany.德国南部患腹泻的哺乳仔猪和断奶仔猪肠道病原体的流行情况
J Vet Med B Infect Dis Vet Public Health. 2001 Mar;48(2):151-9. doi: 10.1046/j.1439-0450.2001.00431.x.
5
Assessment of drugs against Cryptosporidium parvum using a simple in vitro screening method.
FEMS Microbiol Lett. 1999 Sep 15;178(2):227-33. doi: 10.1111/j.1574-6968.1999.tb08681.x.
6
Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus.基于小亚基核糖体RNA基因位点的隐孢子虫寄生虫系统发育分析。
Appl Environ Microbiol. 1999 Apr;65(4):1578-83. doi: 10.1128/AEM.65.4.1578-1583.1999.
7
Molecular and biological characterisation of Cryptosporidium in pigs.
Aust Vet J. 1999 Jan;77(1):44-7. doi: 10.1111/j.1751-0813.1999.tb12428.x.
8
Molecular characterization of Cryptosporidium from various hosts.来自不同宿主的隐孢子虫的分子特征分析。
Parasitology. 1998 Jul;117 ( Pt 1):31-7. doi: 10.1017/s0031182098002765.
9
DNA sequence similarity between California isolates of Cryptosporidium parvum.加利福尼亚微小隐孢子虫分离株之间的DNA序列相似性。
Appl Environ Microbiol. 1998 Apr;64(4):1584-6. doi: 10.1128/AEM.64.4.1584-1586.1998.
10
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.CLUSTAL_X 窗口界面：借助质量分析工具的多序列比对灵活策略。
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82. doi: 10.1093/nar/25.24.4876.
Zotero 插件