Environmental Chemistry and Toxicology Laboratory, Department of Environmental Science, Policy and Management, University of California, Berkeley, California 94720-3112, USA.
J Agric Food Chem. 2011 Apr 13;59(7):2762-9. doi: 10.1021/jf102111s. Epub 2010 Aug 11.
The safe and effective use of pesticides requires knowledge of their mode of action in pests and adverse effects in nontarget organisms coupled with an understanding of their metabolic activation and detoxification. The author and his laboratory colleagues were privileged to observe, participate in, and sometimes influence these developments for the past six decades. This review considers contributions of the Berkeley and Madison laboratories to understanding insecticides acting at voltage-gated sodium and GABA-gated chloride channels and the nicotinic receptor and at serine hydrolases and other targets as well as the action of insecticide synergists and selected herbicides and fungicides. Some of the discoveries gave new probes, radioligands, photoaffinity labeling reagents, and understanding of reactive intermediates that changed the course of pesticide investigations and related areas of science. The importance of coupling mode of action with metabolism and design with serendipity is illustrated with a wide variety of chemotypes.
安全有效地使用农药需要了解其在害虫中的作用模式和对非目标生物的不良影响，同时还需要了解它们的代谢激活和解毒。作者及其实验室同事在过去六十年中有幸观察、参与并有时影响这些发展。本综述考虑了伯克利和麦迪逊实验室在理解作用于电压门控钠和 GABA 门控氯通道以及烟碱受体以及丝氨酸水解酶和其他靶标的杀虫剂，以及杀虫剂增效剂和选定的除草剂和杀菌剂的作用方面的贡献。一些发现提供了新的探针、放射性配体、光亲和标记试剂以及对反应中间体的理解，这些发现改变了农药研究和相关科学领域的进程。通过各种各样的化学类型，说明了将作用模式与代谢和设计与机缘结合起来的重要性。
