Exp Dermatol. 2010 Dec;19(12):1110-2. doi: 10.1111/j.1600-0625.2010.01143.x.
Recent studies suggested that dihydrotestosterone (DHT)-driven alteration in the autocrine and paracrine factors may be a key to androgen-potentiated balding. Also, we recently claimed that DHT-inducible dickkopf-1 (DKK-1) is one of the key factors involved in the androgen-potentiated balding. Here, we investigated whether L-ascorbic acid 2-phosphate (Asc 2-P), a derivative of L-ascorbic acid, could attenuate DHT-induced DKK-1 expression in dermal papilla cells (DPCs) from balding scalp. We observed that DHT-induced DKK-1 mRNA expression was attenuated in the presence of Asc 2-P as examined by RT-PCR analysis. In addition, we found that DHT-induced activation of luciferase reporter activity was significantly repressed when Asc 2-P was added together with DHT. Moreover, Asc 2-P repressed DHT-induced DKK-1 protein expression as examined by enzyme-linked immunosorbent assay (ELISA). Although there will be many hurdles to apply our finding to actual remedies, these results suggest that it would be worthy to evaluate Asc 2-P or its derivatives for the treatment and prevention of androgen-driven balding.
最近的研究表明，二氢睾酮（DHT）驱动的自分泌和旁分泌因子的改变可能是雄激素促进脱发的关键。此外，我们最近声称，DHT 诱导的 dickkopf-1（DKK-1）是参与雄激素促进脱发的关键因素之一。在这里，我们研究了 L-抗坏血酸 2-磷酸（Asc 2-P），即 L-抗坏血酸的衍生物，是否可以减弱脱发头皮的真皮乳头细胞（DPC）中 DHT 诱导的 DKK-1 表达。我们通过 RT-PCR 分析观察到，存在 Asc 2-P 时，DHT 诱导的 DKK-1 mRNA 表达减弱。此外，当 Asc 2-P 与 DHT 一起添加时，我们发现 DHT 诱导的荧光素酶报告基因活性的激活显著受到抑制。此外，通过酶联免疫吸附试验（ELISA）检测，Asc 2-P 抑制了 DHT 诱导的 DKK-1 蛋白表达。尽管将我们的发现应用于实际治疗方法将面临许多障碍，但这些结果表明，评估 Asc 2-P 或其衍生物用于治疗和预防雄激素驱动的脱发将是值得的。
