Department of Immunology, School of Medicine, Kyungpook National University, Daegu 700-422, Korea.
BMB Rep. 2010 Oct;43(10):688-92. doi: 10.5483/BMBRep.2010.43.10.688.
In a previous study, we recently claimed that dihydrotestosterone (DHT)-inducible dickkopf-1 (DKK-1) expression is one of the key factors involved in androgen-potentiated balding. We also demonstrated that L-ascorbic acid 2-phosphate (Asc 2-P) represses DHT-induced DKK-1 expression in cultured dermal papilla cells (DPCs). Here, we investigated whether or not L-threonate could attenuate DHT-induced DKK-1 expression. We observed via RT-PCR analysis and enzyme-linked immunosorbent assay that DHT-induced DKK-1 expression was attenuated in the presence of L-threonate. We also found that DHT-induced activation of DKK-1 promoter activity was significantly repressed by L-threonate. Moreover, a co-culture system featuring outer root sheath (ORS) keratinocytes and DPCs showed that DHT inhibited the growth of ORS cells, which was then significantly reversed by L-threonate. Collectively, these results indicate that L-threonate inhibited DKK-1 expression in DPCs and therefore is a good treatment for the prevention of androgen-driven balding.
在之前的研究中，我们最近声称二氢睾酮（DHT）诱导的 dickkopf-1（DKK-1）表达是参与雄激素性脱发的关键因素之一。我们还证明，L-抗坏血酸 2-磷酸（Asc 2-P）可抑制培养的真皮乳头细胞（DPC）中 DHT 诱导的 DKK-1 表达。在这里，我们研究了 L-苏糖酸是否可以减轻 DHT 诱导的 DKK-1 表达。通过 RT-PCR 分析和酶联免疫吸附试验观察到，L-苏糖酸存在时可减轻 DHT 诱导的 DKK-1 表达。我们还发现，L-苏糖酸显著抑制 DHT 诱导的 DKK-1 启动子活性的激活。此外，具有外根鞘（ORS）角质形成细胞和 DPC 的共培养系统显示，DHT 抑制 ORS 细胞的生长，而 L-苏糖酸则显著逆转了这一作用。综上所述，这些结果表明 L-苏糖酸抑制了 DPC 中的 DKK-1 表达，因此是预防雄激素性脱发的一种很好的治疗方法。
