Pekmezci Erkin, Dundar Cihat, Turkoglu Murat
Department of Dermatology, Gozde Hospital, Malatya, Turkey.
Biota Laboratories, R&D Center, Sancaktepe, Istanbul, Turkey.
Med Arch. 2018 Apr;72(2):136-140. doi: 10.5455/medarh.2018.72.136-140.
Currently while, topical minoxidil and oral finasteride are the only medications approved in androgenetic alopecia (AGA), the cause oriented treatment and immunsupressive treatment are being performed in telogen effluvium (TE) and alopecia areata (AA) respectively. Considering the inflammatory factors in the pathogenesis of these three nonscarring alopecia forms, we have formulated a mixture for topical usage composed of six different herbal extracts (HE) which have already known antiinflammatory and antioxidant features.
In addition to performing the phytochemical analysis of HE, we detected the gene expression level of IL-1α, the crucial hair loss mediator, for the putative efficacy in nonscarring alopecia. Cell proliferation assay was performed by XTT reagent. After determination of non-cytotoxic concentration, HaCaT cells were treated with HE. RNA isolations were carried out from both non-treated and treated cell groups by using TRI-reagent. Gene expressions of IL-1α and as control GAPDH were determined by RT-qPCR analysis.
Results were represented as "IL-1α/GAPDH Fold Change". HE solution caused statistically significant downregulation of IL-1α gene expressions (p<0.0001), compared to untreated control cells. HE treatment ended up with 0.1900 fold change for IL-1α.
IL-1α is a direct growth inhibitory agent in hair follicles and an important actor in the pathogenesis of AGA , TE, and AA. Considering together the vitamins, flavonoids, and trace elements identified in the phytochemical analyses and downregulation of IL-1α in HaCaT cells, our HE may be an auxiliary agent in the therapy of these three nonscarring alopecia forms.
目前，外用米诺地尔和口服非那雄胺是雄激素性脱发（AGA）唯一获批的药物，而休止期脱发（TE）和斑秃（AA）分别采用针对病因的治疗和免疫抑制治疗。考虑到这三种非瘢痕性脱发形式发病机制中的炎症因素，我们配制了一种由六种不同草药提取物（HE）组成的外用混合物，这些提取物具有已知的抗炎和抗氧化特性。
除了对HE进行植物化学分析外，我们检测了关键脱发介质IL-1α的基因表达水平，以确定其在非瘢痕性脱发中的假定疗效。通过XTT试剂进行细胞增殖测定。在确定无细胞毒性浓度后，用HE处理HaCaT细胞。使用TRI试剂从未处理和处理的细胞组中进行RNA分离。通过RT-qPCR分析测定IL-1α和作为对照的GAPDH的基因表达。
结果表示为“IL-1α/GAPDH倍数变化”。与未处理的对照细胞相比，HE溶液导致IL-1α基因表达在统计学上显著下调（p<0.0001）。HE处理后IL-1α的倍数变化为0.1900。
IL-1α是毛囊中的直接生长抑制剂，也是AGA、TE和AA发病机制中的重要因素。综合植物化学分析中鉴定出的维生素、黄酮类化合物和微量元素以及HaCaT细胞中IL-1α的下调情况，我们的HE可能是这三种非瘢痕性脱发形式治疗中的辅助药物。
